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One step purification of GFP-tagged enzymes for enzymatic activity tests

In our experiment we analyzed the enzymatic activity of GFP-DNA methyltransferase 1 (Dnmt1) bound to GFP-Trap® beads and compared the activity of the fluorescent fusion protein to an enzymatically inactive version of Dnmt1 and recombinant Dnmt1.

Content

  • Background
  • Materials and Methods
  • Results
  • Conclusion
  • References/ relevant literature