On-bead digestion protocol
for mass spectrometry following immunoprecipitation with Nano-Traps
The document provides a protocol for on-bead trypsin digestion of ChromoTek GFP-Trap®. The protocol can be adapted for other ChromoTek Nano-Traps.
Learn how you can
- Save time - you don’t need to elute bound proteins
- Keep the protein concentration high - you don’t dilute your sample
- Better retain interacting partners (Co-IP) due to fewer washing steps