On-bead digestion protocol

for mass spectrometry following immunoprecipitation with Nano-Traps

The document provides a protocol for on-bead trypsin digestion of ChromoTek GFP-Trap®. The protocol can be adapted for other ChromoTek Nano-Traps.

Learn how you can 

  • Save time - you don’t need to elute bound proteins

  • Keep the protein concentration high - you don’t dilute your sample

  • Better retain interacting partners (Co-IP) due to fewer washing steps