Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins
Dr. Caroline Rivers, Laboratory for Integrative Neuroscience & Endocrinology, University of Bristol demonstrates the use of ChromoTek’s RFP-Trap in ChIP applications.
In absence of suitable antibodies for an inducible hormone receptor, mCherry has been fused to the N-terminus of the receptor. In order to effectively pull-down the mCherry fusion protein DNA complex, ChromoTek's RFP-Trap was used.
- Materials and Methods
- References/Relevant Literature