Chromatin Immunoprecipitation (ChIP) with RFP-tagged proteins

Dr. Caroline Rivers, Laboratory for Integrative Neuroscience & Endocrinology, University of Bristol demonstrates the use of ChromoTek’s RFP-Trap in ChIP applications.

In absence of suitable antibodies for an inducible hormone receptor, mCherry has been fused to the N-terminus of the receptor. In order to effectively pull-down the mCherry fusion protein DNA complex, ChromoTek's RFP-Trap was used.

Content:

  • Introduction
  • Materials and Methods
  • Reagents
  • Results
  • Conclusion
  • References/Relevant Literature