Blocking in immunofluorescence

What blocking solution should I use for Nano-Secondaries?

Blocking is an essential step during the preparation of a sample for immunofluorescence detection; blocking improves the sensitivity by reducing nonspecific background and therefore increases image quality.

In order to prevent non-specific interactions of the primary and secondary antibody reactive sites of the sample are covered/blocked. In theory, any protein that does not bind to the target antigen can be used as blocking reagent.

Content of the application note:

  • Common blocking buffers
  • Blocking recommendation for immunostaining with Nano-Secondaries
  • Blocking protocols