Blocking in immunofluorescence
What blocking solution should I use for Nano-Secondaries?
Blocking is an essential step during the preparation of a sample for immunofluorescence detection; blocking improves the sensitivity by reducing nonspecific background and therefore increases image quality.
In order to prevent non-specific interactions of the primary and secondary antibody reactive sites of the sample are covered/blocked. In theory, any protein that does not bind to the target antigen can be used as blocking reagent.
Content of the application note:
- Common blocking buffers
- Blocking recommendation for immunostaining with Nano-Secondaries
- Blocking protocols