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GST- & GFP- nanobodies for bead-based protein arrays (BPAs) e.g. Luminex®

This application note reports on a novel approach to generate BPAs combining μ-scale purification of bait proteins combined with site-directed immobilization.

Bait-proteins are produced as GST- or GFP-fusion constructs in bacterial or mammalian cells. They are one-step purified and immobilized from crude lysates using ChromoTeks high affinity tag-specific nanobodies: GFP-Trap® or GST-Trap coupled to color-coded beads (CCBs). Finally, those bait-coupled beads are combined in a protein-array for miniaturized multiplexed GST- and GFP pulldown studies.