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GFP-Trap® capture surface for Biacore assays
This capture system allows an easy and effective isolation and directed immobilization of fluorescent fusion proteins from complex biological samples like prokaryotic or eukaryotic cell extracts. Immobilization of these proteins enables binding studies and kinetic analysis using Biacore systems. With the GFP-Trap capture system valid data on localization, dynamics and mobility of proteins can be directly combined with interaction
analysis using Biacore systems.
This protocol describes mainly how to immobilize and regenerate GFP-Trap.