ChromoBlog

Co-immunoprecipitation troubleshooting

Posted by Dr. Astrid Sitte on Aug 27, 2021 5:21:16 PM

Co-immunoprecipitation (Co-IP) describes the isolation of a protein and its binding partners from a cell extract using a Nanobody or antibody that is bound to beads. The protein, that directly interacts with the Nanobody, or antibody beads is called “bait”. The binding partner that is indirectly precipitated is called “prey”.

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Topics: GFP-Trap, Immunoprecipitation, Co-IP

How to conduct a Co-immunoprecipitation (Co-IP)?

Posted by Dr. Astrid Sitte on Jul 30, 2021 2:39:52 PM

Co-IP describes the immunoprecipitation of a protein of interest and its interacting partners.

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Topics: GFP-Trap, Immunoprecipitation, Co-IP

Flag-tag and 3x Flag-tag: An epitope tag for capture and detection experiments

Posted by Christoph Eckert on Jun 25, 2021 10:35:07 AM

1. What is Flag-Tag?

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Topics: Myc-tag, Spot-Tag, HA-Tag, Flag-Tag

GFP (green fluorescent protein): Properties, origin, specifications, tips

Posted by Christoph Eckert on May 25, 2021 5:13:30 PM

1. What is GFP?

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Topics: GFP, GFP VHH, GFP Nanobody, EGFP

Overview of ChromoTek’s Nanobody-based tools for cancer research

Posted by Christoph Eckert on Apr 28, 2021 5:30:23 PM

Cancer is still a leading cause of mortality. Each year 18 million people worldwide get diagnosed with cancer. The current trend is increasing. This makes cancer research more important.

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Topics: Chromobodies, GFP-Trap, p53/Mdm2, p53/Mdm4, MK2-Trap, Vimentin

Tips and tricks for immunoprecipitation of low abundant proteins

Posted by Dr. Astrid Sitte on Apr 14, 2021 4:03:04 PM

Protein abundance is crucial for immunoprecipitation

During an immunoprecipitation (IP), the protein of interest (POI) is pulled down with a specific antibody or Nanobody conjugated to beads such as agarose or magnetic agarose. Unbound sample content, for example, other proteins, cell debris, and lipids, is removed by washing, while the precipitated protein is enriched on the beads.

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Topics: GFP-Trap, GFP Immunoprecipitation

Overview of the most recent Spot Peptide-Tag & Spot-Nanobody publications

Posted by Dr. Astrid Sitte on Mar 25, 2021 12:46:40 PM

More and more publications are coming up using ChromoTek’s Spot-Tag system, the first peptide-tag specific Nanobody system for universal capture & detection applications.

Interaction of Spot-Nanobody (green) with Spot Peptide

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Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.

Posted by Christoph Eckert on Mar 17, 2021 11:47:58 AM

Myc-tag is a peptide tag derived from the c-Myc protein. The Myc-tag can be used for many capture and detection applications such as immunoprecipitation, immunofluorescence and protein purification.

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Topics: Myc-tag, Spot-Tag, HA-Tag, Flag-Tag

A new Swiss army knife for immunofluorescence: Chimeric heavy chain antibodies

Posted by Klaus Herick on Feb 25, 2021 12:45:00 PM

Nanobody-Fc fusion - An alternative Nanobody format that combines its special epitope binding properties with the detection options for Fc-domains

What are heavy chain antibodies?

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Topics: Immunofluorescence, VHH, Nanobody, mNeonGreen, GFP, TurboGFP, Antibody, Vimentin

Seizing the day: How to save time on your immunoassay experiments

Posted by Dr. Rebecca Northeast (Proteintech) on Feb 3, 2021 10:59:58 AM

When time in the lab is limited, learn how to save precious hours on your immunoprecipitation, immunofluorescence and western blotting experiments.

Due to the current situation with the COVID-19 pandemic, many researchers around the world are faced with restrictions on time and resources in the lab. As we adapt to these changes, the pressure is on to find ways to save time without compromising on results. Proteintech and ChromoTek offer solutions to save you hours on your current immunoassay protocols, helping you achieve publication worthy results in a fast and reliable manner.

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Topics: Immunoprecipitation, Immunofluorescence, GFP, Nano-Secondaries

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