ChromoBlog

Overview of the most recent Spot Peptide-Tag & Spot-Nanobody publications

Posted by Dr. Astrid Sitte on Mar 25, 2021 12:46:40 PM

More and more publications are coming up using ChromoTek’s Spot-Tag system, the first peptide-tag specific Nanobody system for universal capture & detection applications.

Interaction of Spot-Nanobody (green) with Spot Peptide

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Myc-tag: An epitope tag for protein characterization, protein interaction analysis, and purification.

Posted by Christoph Eckert on Mar 17, 2021 11:47:58 AM

Myc-tag is a peptide tag derived from the c-Myc protein. The Myc-tag can be used for many capture and detection applications such as immunoprecipitation, immunofluorescence and protein purification.

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Topics: Myc-tag, Spot-Tag, HA-Tag, Flag-Tag

A new Swiss army knife for immunofluorescence: Chimeric heavy chain antibodies

Posted by Klaus Herick on Feb 25, 2021 12:45:00 PM

Nanobody-Fc fusion - An alternative Nanobody format that combines its special epitope binding properties with the detection options for Fc-domains

What are heavy chain antibodies?

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Topics: Immunofluorescence, VHH, Nanobody, mNeonGreen, GFP, TurboGFP, Antibody, Vimentin

Seizing the day: How to save time on your immunoassay experiments

Posted by Dr. Rebecca Northeast (Proteintech) on Feb 3, 2021 10:59:58 AM

When time in the lab is limited, learn how to save precious hours on your immunoprecipitation, immunofluorescence and western blotting experiments.

Due to the current situation with the COVID-19 pandemic, many researchers around the world are faced with restrictions on time and resources in the lab. As we adapt to these changes, the pressure is on to find ways to save time without compromising on results. Proteintech and ChromoTek offer solutions to save you hours on your current immunoassay protocols, helping you achieve publication worthy results in a fast and reliable manner.

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Topics: Immunoprecipitation, Immunofluorescence, GFP, Nano-Secondaries

Conjugation of fluorescent dyes to Nanobodies

Posted by Dr. Astrid Sitte on Jan 27, 2021 2:01:35 PM

The conjugation of the binding domains of camelid heavy chain antibodies, also known as VHHs or Nanobodies, to fluorescent dyes is generating versatile tools for fluorescence microscopy applications like immunofluorescence (IF) and super resolution microscopy (SRM): The small size Nanobodies provide better penetration into tissue and dense cell compartments, plus the small label to epitope displacement provides higher resolution. In addition, the Nanobodies’ high affinity binding to epitope makes them attractive probes for other assays like ELISA.

ChromoTek, part of Proteintech group, provides multiple fluorescent dye (Alexa Fluor® and ATTO) conjugated Nanobodies that are optimized by application:

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Neuropilin-1 joins ACE2 as SARS-CoV-2 gateaway

Posted by Dr. Rebecca Northeast (Proteintech), Dr. Astrid Sitte on Jan 15, 2021 11:39:14 AM

Using both Proteintech and Chromotek antibodies, Davy and colleagues published a landmark paper in Science late in 2020, detailing how Neuropilin-1 is required for SARS-CoV-2 host cell entry.

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Topics: GFP-Trap, RFP-Trap

V5 Tag: Properties, origin, specifications, antibodies & Nanobodies

Posted by Christoph Eckert on Sep 23, 2020 2:09:32 PM

V5 tag is a short peptide tag for detection and purification of proteins. The V5 tag can be fused/cloned to a recombinant protein and detected in ELISA, flow cytometry, immunoprecipitation, immunofluorescence, and Western blotting with antibodies and Nanobodies.

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Virus proteins are useful!

Posted by Klaus Herick on Sep 23, 2020 1:25:29 PM

 

Parts of them are used as epitope tags

Epitope tags, i.e. short peptide tags are commonly used, versatile, and convenient tools for multiple capture and detection applications including immunofluorescence, immunoprecipitation, affinity purification, and Western blotting.

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Topics: epitope tag

Which beads should I use for my immunoprecipitation?

Posted by Dr. Astrid Sitte on Sep 16, 2020 9:42:57 AM

ChromoTek's Nano-Traps are optimized for the immunoprecipitation (IP) of proteins and their interacting factors. Nano-Traps comprise of a Nanobody/ VHH conjugated to beads. For 3 Nano-Traps, e.g. GFP-Trap®RFP-Trap®, and Spot-Trap®, we offer different types of beads:

  • Agarose beads
  • Magnetic Agarose beads
  • Dynabeads™

Immunoprecipitation with GFP-Trap.
I: Input, FT: Flow-Through, B: Bound

Based on the properties of the different beads (see table), we recommend:

  • Agarose beads for very low background and high binding capacity IP
  • Magnetic Agarose beads for magnetic separation and high binding capacity IP
  • Dynabeads for IP of very large proteins/complexes
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Topics: GFP-Trap, Immunoprecipitation, RFP-Trap, GFP Immunoprecipitation, GFP Nanobody, Spot-Trap, Dynabeads

Characterization of COVID-19 antibodies by Bio-Layer Interferometry using Nano-CaptureLigands

Posted by Dr. Astrid Sitte on Jul 8, 2020 9:10:21 AM

Characterization of antibodies

Essential aspects of how antibodies (Abs) bind to their targets are their affinity and binding kinetics. In order to determine these parameters, different biophysical methods such as Surface Plasmon Resonance (SPR), Bio-Layer Interferometry (BLI), MicroScale Thermophoresis (MST), or Enzyme-linked Immunosorbent Assay (ELISA) can be applied. Some of these methods require the immobilization of the antibody on a surface and the titration of the target antigen to measure the dissociation constant KD and the association and dissociation rates ka and kd (also called on- and off rates kon and koff).

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Topics: Nano-CaptureLigands

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