ChromoBlog

A collaboration between ChromoTek GmbH and Ludwig-Maximilians-University Munich (Germany) has yielded a novel nanobody that sheds new light on an important protein complex called INO80. This work was recently published in the high-impact journal Nature Structural & Molecular Biology (Knoll et al. 2018).

Topics: Nanobody, Crystallisation, Immunoprecipitation

We are happy to present an updated list of scientific publications that have been published based on data generated by Myc-Trap. See detailed list below.

Topics: Nano-Traps, Myc Nanobody, Myc-tag IP, Myc-Trap

Chromotek’s GFP- binding protein, an anti- GFP Nanobody, is a very small and effective tool for binding and visualizing GFP-tagged proteins. In their recent publication, Modi et al. successfully functionalized quantum dots with the GFP- Nanobody (QD GFP- Nanobody). Thus, they created a small GFP- specific label with a very strong fluorescent signal.

Topics: GFP Nanobody, RFP Nanobody, Quantum dots, single particle tracking, Quantum dot Nanobody

TurboGFP is a bright green fluorescent protein used to study protein function, localization and dynamics in cells. Nanobody based research tools allow reproducible biochemical analysis including mass spectrometry and enzyme activity measurements of TurboGFP fusion proteins.

Topics: TurboGFP, TurboGFP immunoprecipitation, Best TurboGFP antibody, TurboGFP Nanobody, Immunoprecipitation

You may have noticed the recent publication “A peptide tag-specific nanobody enables high-quality labeling for STORM imaging” of Virant et al (2018) in Nature Communications doi: 10.1038/s41467-018-03191-2, where for the first time a peptide-tag specific Nanobody was applied in dSTORM imaging: The authors have described and discussed the performance of a BC2 peptide-tag specific Nanobody to image BC2 peptide tag fusion proteins in super resolution microscopy, i.e. dSTORM. This Blog features that BC2 peptide-tag specific Nanobody, which ChromoTek markets under the names Spot-Label® and Spot-Trap®. In addition, this Blog figures the significantly improved version of the BC2 peptide -tag, which is called Spot-Tag®.

Topics: Spot-Tag, Spot-Trap, Spot-Label, Capture/detection System, Spot Nanobody, Nanobody, Spot immunofluorescence, Spot super resolution microscopy, Spot STED, BC2tag

What makes on-bead digestion favorable?

Just pull down your protein of interest with immobilized nanobodies, also termed V_HHs or single domain antibodies. Then follow the on-bead digestion protocol (see below) and submit the digest to your core facility for effective mass spectrometer analysis of (co-) precipitated proteins.

Topics: GFP Immunoprecipitation, GFP Nanobody, GFP VHH, GFP mass spec, on-bead digestion, GFP-Trap

Life science laboratories apply green fluorescent proteins (GFP) to study protein localization, interaction and dynamics in fluorescence microscopy. Immunoprecipitation (IP), mass spectrometry (MS), co-immunoprecipitation (Co-IP) and/or affinity purification investigate more aspects including posttranslational modifications (PTMs), DNA binding, and protein-protein interaction. Here, we compare two different antibody systems for immunoprecipitation of GFP-fusion proteins: GFP-Trap and anti-GFP IgG antibody

Topics: GFP, GFP Immunoprecipitation, GFP-Trap, Best GFP antibody for IP, GFP VHH, GFP Nanobody, Best antibody for immunoprecipitation

The ChromoTek Histone-Chromobody®* is a novel probe to visualize endogenous histone H2A-H2B heterodimer in live cells:

Topics: Histone, Histone Chromobody, live cell microscopy, live cell imaging, Nucleosome, Chromatin Marker

Recently a new bright monomeric yellow-green fluorescent protein has been published, which is called mNeonGreen. This protein has already been frequently used for mainly microscopic applications in both wide-field microscopy and super resolution microscopy. What is mNeonGreen all about?

Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen immunofluorescence, mNeonGreen immunoprecipitation, mNeonGreen Western blot, mNeonGreen VHH, GFP, mNeonGreen sequence

ChromoTek is proud to introduce new tools for mNeonGreen fusion proteins: 

• anti-mNeonGreen mouse monoclonal antibody 32F6 for immunofluorescence (IF) and Western blot (WB) 
• mNeonGreen-Trap for immunoprecipitation (IP) of mNeonGreen fusion proteins. The mNeonGreen-Trap is based on an anti-mNeonGreen VHH (mNeonGreen binding protein)

Click here and request a free sample:

Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen immunoprecipitation, mNeonGreen immunofluorescence, mNeonGreen Western blot, mNeonGreen VHH, mNeonGreen-Trap