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GFP- Nanobodies and RFP- Nanobodies conjugated to quantum dots

Posted by Christoph Eckert on Jul 12, 2018 8:39:33 AM

Chromotek’s GFP- binding protein, an anti- GFP Nanobody, is a very small and effective tool for binding and visualizing GFP-tagged proteins. In their recent publication, Modi et al. successfully functionalized quantum dots with the GFP- Nanobody (QD GFP- Nanobody). Thus, they created a small GFP- specific label with a very strong fluorescent signal.

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Topics: GFP Nanobody, RFP Nanobody, Quantum dots, single particle tracking, Quantum dot Nanobody

TurboGFP: Properties, Sequence, MW, origin

Posted by Christoph Eckert on Jul 3, 2018 1:23:20 PM

TurboGFP is a bright green fluorescent protein used to study protein function, localization and dynamics in cells. Nanobody based research tools allow reproducible biochemical analysis including mass spectrometry and enzyme activity measurements of TurboGFP fusion proteins.

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Topics: Immunoprecipitation, TurboGFP, TurboGFP immunoprecipitation, Best TurboGFP antibody, TurboGFP Nanobody

First Peptide-tag specific Nanobody applied in super resolution microscopy (SRM)

Posted by Klaus Herick on Mar 2, 2018 11:02:00 AM

You may have noticed the recent publication “A peptide tag-specific nanobody enables high-quality labeling for STORM imaging” of Virant et al (2018) in Nature Communications doi: 10.1038/s41467-018-03191-2, where for the first time a peptide-tag specific Nanobody was applied in dSTORM imaging: The authors have described and discussed the performance of a BC2 peptide-tag specific Nanobody to image BC2 peptide tag fusion proteins in super resolution microscopy, i.e. dSTORM. This Blog features that BC2 peptide-tag specific Nanobody, which ChromoTek markets under the names Spot-Label® and Spot-Trap®. In addition, this Blog figures the significantly improved version of the BC2 peptide -tag, which is called Spot-Tag®.

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Topics: Nanobody, Spot-Tag, Capture/detection System, Spot Nanobody, Spot-Trap, Spot-Label, Spot super resolution microscopy, Spot STED, Spot immunofluorescence, BC2tag

On-bead digestion protocol: From immunoprecipitation with nanobodies to MS analysis

Posted by Christoph Eckert on Sep 15, 2017 3:52:48 PM

What makes on-bead digestion favorable?

Just pull down your protein of interest with immobilized nanobodies, also termed VHHs or single domain antibodies. Then follow the on-bead digestion protocol (see below) and submit the digest to your core facility for effective mass spectrometer analysis of (co-) precipitated proteins.

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Topics: GFP-Trap, GFP Immunoprecipitation, GFP VHH, GFP Nanobody, on-bead digestion, GFP mass spec

The best anti-GFP antibody for immunoprecipitation: GFP-Trap

Posted by Christoph Eckert on Sep 7, 2017 1:05:34 PM

Life science laboratories apply green fluorescent proteins (GFP) to study protein localization, interaction and dynamics in fluorescence microscopy. Immunoprecipitation (IP), mass spectrometry (MS), co-immunoprecipitation (Co-IP) and/or affinity purification investigate more aspects including posttranslational modifications (PTMs), DNA binding, and protein-protein interaction. Here, we compare two different antibody systems for immunoprecipitation of GFP-fusion proteins: GFP-Trap and anti-GFP IgG antibody

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Topics: GFP-Trap, GFP, GFP Immunoprecipitation, Best GFP antibody for IP, Best antibody for immunoprecipitation, GFP VHH, GFP Nanobody

Visualize Histones in live cells: Histone-Chromobody

Posted by Christoph Eckert on Aug 3, 2017 1:22:32 PM

The ChromoTek Histone-Chromobody®* is a novel probe to visualize endogenous histone H2A-H2B heterodimer in live cells:

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Topics: live cell imaging, Histone Chromobody, live cell microscopy, Nucleosome, Chromatin Marker, Histone

mNeonGreen: A green fluorescent protein that is not a GFP variant

Posted by Christoph Eckert on Jul 19, 2017 8:20:46 AM

Recently a new bright monomeric yellow-green fluorescent protein has been published, which is called mNeonGreen. This protein has already been frequently used for mainly microscopic applications in both wide-field microscopy and super resolution microscopy. What is mNeonGreen all about?

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Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen Western blot, mNeonGreen VHH, mNeonGreen immunoprecipitation, mNeonGreen immunofluorescence, GFP, mNeonGreen sequence

New anti-mNeonGreen antibody & Nano-Trap for Immunofluorescence, Western Blot & Immunoprecipitation

Posted by Christoph Eckert on Jul 13, 2017 11:29:11 AM

ChromoTek is proud to introduce new tools for mNeonGreen fusion proteins: 

Click here and request a free sample:

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Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen Western blot, mNeonGreen VHH, mNeonGreen-Trap, mNeonGreen immunoprecipitation, mNeonGreen immunofluorescence

Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins

Posted by Klaus Herick on Jul 6, 2017 1:30:48 PM

Multiple literature references for GST-Trap


This article provides an overview of scientific publications that reference the ChromoTek GST-Trap superior binding performance for various applications. These include immunoprecipitation (IP), Co-IP of Glutathione S-Transferase (GST)-fusion proteins, and Luminex bead assays of GST-tagged and GFP-tagged proteins. Besides, usages for depletion of GST (glutathione sepharose)  after cleavage from recombinant fusion proteins and affinity purification of GST-tagged proteins are possible.

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Topics: Immunoprecipitation, GST, affinity resin, GST immunoprecipitation, references, affinity purification, GST pulldown

ChromoTek Nano-Booster for super resolution microscopy is featured at Biocompare

Posted by Klaus Herick on Jun 2, 2017 4:21:49 PM

ChromoTek’s Marketing Manager Dr. Christoph Eckert has been interviewed by Biocompare, the buyers’ guide for life scientists, on labels for super resolution microscopy.

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Topics: GFP-Booster, RFP-Booster, Super resolution microscopy, VHH, Nanobody

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