ChromoBlog

An extract of the published literature since 2016

This blog provides references from the last 4 years in virus research using GFP. Most publications report how immunoprecipitation (IP)/Co-IP of GFP-fusions was conducted to identify host cell binding partners of virus proteins. In addition, mass spectrometry analysis and functional assays have been performed.

Christian Linke-Winnebeck^[1]; Michael Metterlein^[1]; Larisa Yurlova^[1]; Sabrina Wendler^[1]; Simge Yüz^[2]; Björn Tränkle^[3]; Jacqueline Boger^[1]; Tanja Ertl^[1]; Felix Hartlepp^[1]

GFP-Trap^®Multiwell Plate for convenient high throughput analysis of protein interactions. It consists of our anti-GFP VHH immobilized in multiwell plates. The GFP-Trap Multiwell Plate is optimized for immunoprecipitation (IP), IP-MS, and ELISA. Here you can find the protocol for sandwich ELISA und immunoprecipitation (IP) with GFP-Trap

Introduction of Affinity vs. Specificity

Introduction

A high background from unspecific binding of proteins is a common problem in immunoprecipitation (IP). In this blog, the origin of background and different optimization strategies are discussed. Furthermore, it is shown how you can achieve a low background when using the ChromoTek GFP-Trap^®, a ready to use reagent for IP of a GFP-tagged proteins.

Estimated reading time:  3 minutes

The pull-down of proteins is an established technology. It can be difficult, particularly when the protein of interest is expressed at low levels. Here we discuss 5 tips that help you to improve your IP results.

Affinity tags are valuable tools for immunoprecipitation (IP). Fused to the protein of interest, they enable proteome-wide identification of interaction partners by mass spectrometry.

New iST Nano-Trap kits for immunoprecipitation (IP) and sample preparation for mass spectrometry (MS) in just 4 easy steps:

GFP-Booster and RFP-Booster conjugated to Alexa Fluor® 488, 568 and 647:

• Stronger signal in confocal and standard fluorescence microscopy
• More options in super-resolution microscopy including STORM and MINFLUX
• Constant degree of labeling (DOL = 2 dyes per Booster) for higher resolution
• More than 95% labeling efficiency