ChromoBlog

Virus research using GFP

Posted by Dr. Astrid Sitte on Mar 26, 2020 1:05:30 PM

An extract of the published literature since 2016

This blog provides references from the last 4 years in virus research using GFP. Most publications report how immunoprecipitation (IP)/Co-IP of GFP-fusions was conducted to identify host cell binding partners of virus proteins. In addition, mass spectrometry analysis and functional assays have been performed.

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Topics: GFP

Abstract on Nano-BiTEs: Bispecific T cell engagers based on nanobodies

Posted by Christian Linke-Winnebeck on Mar 23, 2020 11:13:01 AM

Christian Linke-Winnebeck[1]; Michael Metterlein[1]; Larisa Yurlova[1]; Sabrina Wendler[1]; Simge Yüz[2]; Björn Tränkle[3]; Jacqueline Boger[1]; Tanja Ertl[1]; Felix Hartlepp[1]

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Topics: Nano-BiTEs

GFP-Trap in multiwell plates

Posted by Christoph Eckert on Mar 19, 2020 2:10:56 PM

GFP-Trap®Multiwell Plate for convenient high throughput analysis of protein interactions. It consists of our anti-GFP VHH immobilized in multiwell plates. The GFP-Trap Multiwell Plate is optimized for immunoprecipitation (IP), IP-MS, and ELISA. Here you can find the protocol for sandwich ELISA und immunoprecipitation (IP) with GFP-Trap

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Topics: GFP Immunoprecipitation

What is antibody specificity and affinity? And why is it important for immunoprecipitation?

Posted by Dr. Astrid Sitte on Mar 12, 2020 5:17:58 PM

Introduction of Affinity vs. Specificity

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Topics: Immunoprecipitation

How to obtain a low background in immunoprecipitation assays

Posted by Dr. Astrid Sitte on Mar 10, 2020 2:02:57 PM

Introduction

A high background from unspecific binding of proteins is a common problem in immunoprecipitation (IP). In this blog, the origin of background and different optimization strategies are discussed. Furthermore, it is shown how you can achieve a low background when using the ChromoTek GFP-Trap®, a ready to use reagent for IP of a GFP-tagged proteins.

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Topics: GFP-Trap, Immunoprecipitation

Immunoprecipitation without additional bands

Posted by Dr. Astrid Sitte on Mar 6, 2020 3:03:09 PM

Estimated reading time:  3 minutes

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Topics: Immunoprecipitation

5 Tips for Successful Immunoprecipitations (IP)

Posted by Klaus Herick on Mar 3, 2020 3:15:19 PM

The pull-down of proteins is an established technology. It can be difficult, particularly when the protein of interest is expressed at low levels. Here we discuss 5 tips that help you to improve your IP results.

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Topics: Immunoprecipitation, GFP Immunoprecipitation

Multiple kits for streamlined affinity purification mass spectrometry (AP-MS)

Posted by Dr. Fabian Hosp (PreOmics) on Feb 24, 2020 10:13:00 AM

Affinity tags are valuable tools for immunoprecipitation (IP). Fused to the protein of interest, they enable proteome-wide identification of interaction partners by mass spectrometry.

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Topics: Immunoprecipitation, Mass spec

Mass spec-compatible immunoprecipiation for GFP, mNeonGreen, Myc, RFP, Spot, and TurboGFP

Posted by Christoph Eckert on Feb 20, 2020 1:12:28 PM

New iST Nano-Trap kits for immunoprecipitation (IP) and sample preparation for mass spectrometry (MS) in just 4 easy steps:

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Topics: Immunoprecipitation, Mass spec, Myc-tag, VHH, Nanobody, mNeonGreen, GFP, Spot-Tag, TurboGFP, RFP

GFP and RFP-Booster for better immunofluorescence imaging

Posted by Christoph Eckert on Jan 29, 2020 5:37:07 PM

GFP-Booster and RFP-Booster conjugated to Alexa Fluor® 488, 568 and 647:

  • Stronger signal in confocal and standard fluorescence microscopy
  • More options in super-resolution microscopy including STORM and MINFLUX
  • Constant degree of labeling (DOL = 2 dyes per Booster) for higher resolution
  • More than 95% labeling efficiency
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Topics: GFP-Booster, RFP-Booster, GFP Nanobody, RFP Nanobody, EGFP

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