A collaboration between ChromoTek GmbH and Ludwig-Maximilians-University Munich (Germany) has yielded a novel nanobody that sheds new light on an important protein complex called INO80. This work was recently published in the high-impact journal Nature Structural & Molecular Biology (Knoll et al. 2018).
Christian Linke-Winnebeck
Recent Posts
ChromoTek’s Nanobody helps LMU scientists in their quest to understand a giant protein complex
Posted by Christian Linke-Winnebeck on Sep 20, 2018 3:42:47 PM
Topics: Nanobody, Crystallisation, Immunoprecipitation
5 Tips for better immunoprecipitation (IP)
Posted by Christian Linke-Winnebeck on Jul 21, 2016 1:10:15 PM
Pull-down of proteins can be difficult, particularly when they are expressed at low levels. Here we present 5 tips, which will considerably improve your IP results.
- Protein concentration matters
The higher the protein concentration, the higher the IP yield. Try to use a concentration as high as possible.It makes a significant difference if the concentration of your protein during IP is 1 nM (low protein expression level), about 50 nM (intracellular endogenous protein expression level, will be diluted by buffer for IP) or 1000 nM (high protein concentration).
Topics: Nano-Trap, Immunoprecipitation, Co-IP, GST pulldown, MBP pulldown
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