ChromoBlog

Christoph Eckert

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Working with green fluorescent proteins: Tools and properties

Posted by Christoph Eckert on Feb 7, 2019 1:18:14 PM

Jellyfish Green Fluorescent Protein (GFP) and its derivatives are still the most frequently used fluorescent proteins in biomedical research. Recently, additional green fluorescent proteins have been discovered in higher animals such as crustaceans and lancelets. These FPs share a common fold, but diverge widely in their primary sequence. Thus, they require novel, dedicated antibody research tools. Here is an overview about EGFP (the most commonly used GFP derivative), TurboGFP and mNeonGreen.

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Topics: TurboGFP immunoprecipitation, GFP Immunoprecipitation, mNeonGreen immunoprecipitation, EGFP, GFP, mNeonGreen, TurboGFP, Nanobody

GFP- Nanobodies and RFP- Nanobodies conjugated to quantum dots

Posted by Christoph Eckert on Jul 12, 2018 8:39:33 AM

Chromotek’s GFP- binding protein, an anti- GFP Nanobody, is a very small and effective tool for binding and visualizing GFP-tagged proteins. In their recent publication, Modi et al. successfully functionalized quantum dots with the GFP- Nanobody (QD GFP- Nanobody). Thus, they created a small GFP- specific label with a very strong fluorescent signal.

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Topics: GFP Nanobody, RFP Nanobody, Quantum dots, single particle tracking, Quantum dot Nanobody

TurboGFP: Properties, Sequence, MW, origin

Posted by Christoph Eckert on Jul 3, 2018 1:23:20 PM

TurboGFP is a bright green fluorescent protein used to study protein function, localization and dynamics in cells. Nanobody based research tools allow reproducible biochemical analysis including mass spectrometry and enzyme activity measurements of TurboGFP fusion proteins.

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Topics: TurboGFP, TurboGFP immunoprecipitation, Best TurboGFP antibody, TurboGFP Nanobody, Immunoprecipitation

On-bead digestion protocol: From immunoprecipitation with nanobodies to MS analysis

Posted by Christoph Eckert on Sep 15, 2017 3:52:48 PM

What makes on-bead digestion favorable?

Just pull down your protein of interest with immobilized nanobodies, also termed VHHs or single domain antibodies. Then follow the on-bead digestion protocol (see below) and submit the digest to your core facility for effective mass spectrometer analysis of (co-) precipitated proteins.

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Topics: GFP Immunoprecipitation, GFP Nanobody, GFP VHH, GFP mass spec, on-bead digestion, GFP-Trap

The best anti-GFP antibody for immunoprecipitation: GFP-Trap

Posted by Christoph Eckert on Sep 7, 2017 1:05:34 PM

Life science laboratories apply green fluorescent proteins (GFP) to study protein localization, interaction and dynamics in fluorescence microscopy. Immunoprecipitation (IP), mass spectrometry (MS), co-immunoprecipitation (Co-IP) and/or affinity purification investigate more aspects including posttranslational modifications (PTMs), DNA binding, and protein-protein interaction. Here, we compare two different antibody systems for immunoprecipitation of GFP-fusion proteins: GFP-Trap and anti-GFP IgG antibody

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Topics: GFP, GFP Immunoprecipitation, GFP-Trap, Best GFP antibody for IP, GFP VHH, GFP Nanobody, Best antibody for immunoprecipitation

Visualize Histones in live cells: Histone-Chromobody

Posted by Christoph Eckert on Aug 3, 2017 1:22:32 PM

The ChromoTek Histone-Chromobody®* is a novel probe to visualize endogenous histone H2A-H2B heterodimer in live cells:

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Topics: Histone, Histone Chromobody, live cell microscopy, live cell imaging, Nucleosome, Chromatin Marker

mNeonGreen: A green fluorescent protein that is not a GFP variant

Posted by Christoph Eckert on Jul 19, 2017 8:20:46 AM

Recently a new bright monomeric yellow-green fluorescent protein has been published, which is called mNeonGreen. This protein has already been frequently used for mainly microscopic applications in both wide-field microscopy and super resolution microscopy. What is mNeonGreen all about?

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Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen immunofluorescence, mNeonGreen immunoprecipitation, mNeonGreen Western blot, mNeonGreen VHH, GFP, mNeonGreen sequence

New anti-mNeonGreen antibody & Nano-Trap for Immunofluorescence, Western Blot & Immunoprecipitation

Posted by Christoph Eckert on Jul 13, 2017 11:29:11 AM

ChromoTek is proud to introduce new tools for mNeonGreen fusion proteins: 

Click here and request a free sample:

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Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen immunoprecipitation, mNeonGreen immunofluorescence, mNeonGreen Western blot, mNeonGreen VHH, mNeonGreen-Trap

Immunoprecipitation of Myc-tagged proteins – How it works

Posted by Christoph Eckert on May 26, 2017 1:57:07 PM

ChromoTek Myc-Trap® is an effectiveness optimized tool for immunoprecipitation (IP/Co-IP) and affinity purification of Myc-tagged proteins. Depending on whether you use a single Myc-tag, i.e. 1xMyc (EQKLISEEDL) or a double Myc-tag fused to the protein of interest, i.e. 2xMyc (EQKLISEEDLEQKLISEEDL) you can adjust your experiment. Here, we introduce the epitope of Myc-Trap to discuss its performance for afore mentioned applications. 

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Topics: Immunoprecipitation, Myc-tag, Myc-tag IP

Analyze protein interactions live in mammalian cells

Posted by Christoph Eckert on May 16, 2017 4:18:31 PM

  • Do you have GFP or RFP protein constructs and/or libraries?
  • Do you want to screen agonists or antagonists?
  • Do you want to save time and start your experiment without method set-up?

Just do it. All you need is the ChromoTek F2H Kit Basic: Transfect the bait and the prey plasmids with the Platform Reagent into the F2H cells. Image and quantify your results already at the next day.

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Topics: F2H assay, PPI

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