ChromoBlog

Jellyfish Green Fluorescent Protein (GFP) and its derivatives are still the most frequently used fluorescent proteins in biomedical research. Recently, additional green fluorescent proteins have been discovered in higher animals such as crustaceans and lancelets. These FPs share a common fold, but diverge widely in their primary sequence. Thus, they require novel, dedicated antibody research tools. Here is an overview about EGFP (the most commonly used GFP derivative), TurboGFP and mNeonGreen.

Topics: TurboGFP immunoprecipitation, GFP Immunoprecipitation, mNeonGreen immunoprecipitation, EGFP, GFP, mNeonGreen, TurboGFP, Nanobody

Chromotek’s GFP- binding protein, an anti- GFP Nanobody, is a very small and effective tool for binding and visualizing GFP-tagged proteins. In their recent publication, Modi et al. successfully functionalized quantum dots with the GFP- Nanobody (QD GFP- Nanobody). Thus, they created a small GFP- specific label with a very strong fluorescent signal.

Topics: GFP Nanobody, RFP Nanobody, Quantum dots, single particle tracking, Quantum dot Nanobody

TurboGFP is a bright green fluorescent protein used to study protein function, localization and dynamics in cells. Nanobody based research tools allow reproducible biochemical analysis including mass spectrometry and enzyme activity measurements of TurboGFP fusion proteins.

Topics: TurboGFP, TurboGFP immunoprecipitation, Best TurboGFP antibody, TurboGFP Nanobody, Immunoprecipitation

What makes on-bead digestion favorable?

Just pull down your protein of interest with immobilized nanobodies, also termed V_HHs or single domain antibodies. Then follow the on-bead digestion protocol (see below) and submit the digest to your core facility for effective mass spectrometer analysis of (co-) precipitated proteins.

Topics: GFP Immunoprecipitation, GFP Nanobody, GFP VHH, GFP mass spec, on-bead digestion, GFP-Trap

Life science laboratories apply green fluorescent proteins (GFP) to study protein localization, interaction and dynamics in fluorescence microscopy. Immunoprecipitation (IP), mass spectrometry (MS), co-immunoprecipitation (Co-IP) and/or affinity purification investigate more aspects including posttranslational modifications (PTMs), DNA binding, and protein-protein interaction. Here, we compare two different antibody systems for immunoprecipitation of GFP-fusion proteins: GFP-Trap and anti-GFP IgG antibody

Topics: GFP, GFP Immunoprecipitation, GFP-Trap, Best GFP antibody for IP, GFP VHH, GFP Nanobody, Best antibody for immunoprecipitation

The ChromoTek Histone-Chromobody®* is a novel probe to visualize endogenous histone H2A-H2B heterodimer in live cells:

Topics: Histone, Histone Chromobody, live cell microscopy, live cell imaging, Nucleosome, Chromatin Marker

Recently a new bright monomeric yellow-green fluorescent protein has been published, which is called mNeonGreen. This protein has already been frequently used for mainly microscopic applications in both wide-field microscopy and super resolution microscopy. What is mNeonGreen all about?

Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen immunofluorescence, mNeonGreen immunoprecipitation, mNeonGreen Western blot, mNeonGreen VHH, GFP, mNeonGreen sequence

ChromoTek is proud to introduce new tools for mNeonGreen fusion proteins: 

• anti-mNeonGreen mouse monoclonal antibody 32F6 for immunofluorescence (IF) and Western blot (WB) 
• mNeonGreen-Trap for immunoprecipitation (IP) of mNeonGreen fusion proteins. The mNeonGreen-Trap is based on an anti-mNeonGreen VHH (mNeonGreen binding protein)

Click here and request a free sample:

Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen immunoprecipitation, mNeonGreen immunofluorescence, mNeonGreen Western blot, mNeonGreen VHH, mNeonGreen-Trap

ChromoTek Myc-Trap® is an effectiveness optimized tool for immunoprecipitation (IP/Co-IP) and affinity purification of Myc-tagged proteins. Depending on whether you use a single Myc-tag, i.e. 1xMyc (EQKLISEEDL) or a double Myc-tag fused to the protein of interest, i.e. 2xMyc (EQKLISEEDLEQKLISEEDL) you can adjust your experiment. Here, we introduce the epitope of Myc-Trap to discuss its performance for afore mentioned applications. 

Topics: Immunoprecipitation, Myc-tag, Myc-tag IP

• Do you have GFP or RFP protein constructs and/or libraries?
• Do you want to screen agonists or antagonists?
• Do you want to save time and start your experiment without method set-up?

Just do it. All you need is the ChromoTek F2H Kit Basic: Transfect the bait and the prey plasmids with the Platform Reagent into the F2H cells. Image and quantify your results already at the next day.

Topics: F2H assay, PPI