ChromoBlog

Christoph Eckert

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SNAP-tag and CLIP-tag

Posted by Christoph Eckert on May 7, 2020 9:34:17 AM

SNAP-tag and CLIP-tag are self-labeling protein tags. The SNAP-tag protein and the CLIP-tag protein can be fused to a protein of interest (POI) and used for cellular and biochemical analysis.

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Topics: VHH, Nanobody, SNAP

HaloTag

Posted by Christoph Eckert on May 5, 2020 2:47:26 PM

HaloTag is a protein tag. The HaloTag protein can be fused to your protein of interest (POI) which enables it for cellular and biochemical analysis. It can also be used for live cell imaging.

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Topics: VHH, Nanobody

GFP-Trap in multiwell plates

Posted by Christoph Eckert on Mar 19, 2020 2:10:56 PM

GFP-Trap®Multiwell Plate for convenient high throughput analysis of protein interactions. It consists of our anti-GFP VHH immobilized in multiwell plates. The GFP-Trap Multiwell Plate is optimized for immunoprecipitation (IP), IP-MS, and ELISA. Here you can find the protocol for sandwich ELISA und immunoprecipitation (IP) with GFP-Trap

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Topics: GFP Immunoprecipitation

Mass spec-compatible immunoprecipiation for GFP, mNeonGreen, Myc, RFP, Spot, and TurboGFP

Posted by Christoph Eckert on Feb 20, 2020 1:12:28 PM

New iST Nano-Trap kits for immunoprecipitation (IP) and sample preparation for mass spectrometry (MS) in just 4 easy steps:

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Topics: Immunoprecipitation, Mass spec, Myc-tag, VHH, Nanobody, mNeonGreen, GFP, Spot-Tag, TurboGFP, RFP

GFP and RFP-Booster for better immunofluorescence imaging

Posted by Christoph Eckert on Jan 29, 2020 5:37:07 PM

GFP-Booster and RFP-Booster conjugated to Alexa Fluor® 488, 568 and 647:

  • Stronger signal in confocal and standard fluorescence microscopy
  • More options in super-resolution microscopy including STORM and MINFLUX
  • Constant degree of labeling (DOL = 2 dyes per Booster) for higher resolution
  • More than 95% labeling efficiency
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Topics: GFP-Booster, RFP-Booster, GFP Nanobody, RFP Nanobody, EGFP

GFP-Trap Dynabeads: Most cited GFP-Nanobody conjugated to Dynabeads

Posted by Christoph Eckert on Jan 17, 2020 11:27:30 AM

GFP-Trap® Dynabeads is optimized for the immunoprecipitation (IP) of large GFP-tagged proteins and for Co-IP of protein complexes. It consists of ChromoTek’s established anti-GFP Nanobody conjugated to Dynabeads™. Hence, also GFP-Trap Dynabeads has the very high affinity of 1 pM like GFP-Trap Agarose and Magnetic Agarose.

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Topics: GFP Immunoprecipitation, GFP Nanobody, Dynabeads

Tags for affinity purification

Posted by Christoph Eckert on Nov 18, 2019 2:56:52 PM

Affinity tags are very useful tools for protein purification. Fused to the protein of interest, they streamline the purification process by binding to a tag-specific resin. Obviously, tag selection is an important step as the purification tag can affect expression level, solubility, facilitate correct folding, protect from proteolysis, and re-direct proteins to a cellular compartment. In addition, the purification tag determines the affinity resin used.

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Bispecific T cell engagers

Posted by Christoph Eckert on Sep 13, 2019 1:04:52 PM
Bispecific T cell engagers are bispecific antibodies designed to link T cells with cancer cells. The simultaneous binding of the bispecific antibody to T cell and tumor cell initiates the activation of the T cell. For this purpose, the bispecific antibody carries two antigen binding domains against the T cell (often CD3 T cell receptor) and against a cell surface protein of the tumor cell.

ChromoTek offers two bispecific T cell engagers:

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Topics: VHH, Nanobody, Bispecific, T cell

iCLIP for the thorough analysis of mRNA:protein interactions

Posted by Christoph Eckert on Aug 14, 2019 11:41:21 AM

Crystal structure of the anti-GFP VHH-Green Fluorescent Protein complex.
The GFP Nanobody is displayed blue and the GFP in green color.

UV crosslinking techniques are the method of choice for a comprehensive analysis of in-vivo-mRNA targets of an RNA-binding protein (RBP). In the recent publication of Olgeiser et al. (2019), the authors applied individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) to study fungal mRNA transport. For this approach, they have used strains expressing GFP-tagged versions of the two RBPs Grp1 and Rrm4; an optimized protocol was developed to uncover that Grp1 and Rrm4 conjointly bind thousands of shared target messenger ribonucleoproteins (mRNPs) in the fungus U. maydis. The protein:RNA complexes were immunoprecipitated in a multiple detergent containing buffer using ChromoTek’s GFP-Trap Magnetic Agarose. This is a transcriptome‐wide view to an endosomal mRNA transport machinery.

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Topics: GFP, iCLIP

Working with green fluorescent proteins: Tools and properties

Posted by Christoph Eckert on Feb 7, 2019 1:18:14 PM

Jellyfish Green Fluorescent Protein (GFP) and its derivatives are still the most frequently used fluorescent proteins in biomedical research. Recently, additional green fluorescent proteins have been discovered in higher animals such as crustaceans and lancelets. These FPs share a common fold, but diverge widely in their primary sequence. Thus, they require novel, dedicated antibody research tools. Here is an overview about EGFP (the most commonly used GFP derivative), TurboGFP and mNeonGreen.

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Topics: Nanobody, mNeonGreen, mNeonGreen immunoprecipitation, GFP, GFP Immunoprecipitation, TurboGFP, TurboGFP immunoprecipitation, EGFP

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