We are happy to present an updated list of scientific publications that have been published based on data generated by Myc-Trap. See detailed list below.
You may have noticed the recent publication “A peptide tag-specific nanobody enables high-quality labeling for STORM imaging” of Virant et al (2018) in Nature Communications doi: 10.1038/s41467-018-03191-2, where for the first time a peptide-tag specific Nanobody was applied in dSTORM imaging: The authors have described and discussed the performance of a BC2 peptide-tag specific Nanobody to image BC2 peptide tag fusion proteins in super resolution microscopy, i.e. dSTORM. This Blog features that BC2 peptide-tag specific Nanobody, which ChromoTek markets under the names Spot-Label® and Spot-Trap®. In addition, this Blog figures the significantly improved version of the BC2 peptide -tag, which is called Spot-Tag®.
Multiple literature references for GST-Trap
This article provides an overview of scientific publications that reference the ChromoTek GST-Trap superior binding performance for various applications. These include immunoprecipitation (IP), Co-IP of Glutathione S-Transferase (GST)-fusion proteins, and Luminex bead assays of GST-tagged and GFP-tagged proteins. Besides, usages for depletion of GST (glutathione sepharose) after cleavage from recombinant fusion proteins and affinity purification of GST-tagged proteins are possible.
ChromoTek’s Marketing Manager Dr. Christoph Eckert has been interviewed by Biocompare, the buyers’ guide for life scientists, on labels for super resolution microscopy.
Practical considerations and instructions
The ChromoTek Myc-Trap is an affinity resin that consists of Myc-binding protein derived from an Alpaca single domain antibody, also called anti-Myc VHH or “anti-Myc nanobody”, which is coupled to agarose and magnetic agarose beads. The Myc-Trap provides advantages over conventional IgG antibodies when applied in immunoprecipitation (IP) and affinity purification experiments:
Although traditional IgG antibodies are often used for immunoprecipitation and protein interaction analysis, GFP-binding protein (GBP, ChromoTek gt-250) and other ChromoTek VHHs (https://www.chromotek.com/about-us/the-alpaca-antibody-advantage/) easily outcompete those under challenging conditions such as elevated temperatures, varying pH or high denaturant concentrations. In fact, GBP tightly binds GFP-fusion proteins even in 8 M urea at 51 °C, which sets a new limit in protein complex stability, virtually unrivalled by any other capture molecule protein tag pair.
On a personal note, we – ChromoTek - like to state a few words on the quality of our camelid VHH or heavy chain antibodies (also known as “nanobodies”).
Topics: Antibody Quality
ChromoTek meets researchers at their institutes in Germany: From October 27 to 30, 2015 our senior scientists Dr. Andrea Buchfellner and Dr. Larisa Yurlova will be on a 2.000 km road show and provide scientific seminar lecture series. They will deliver presentations on two topics as requested by local scientists:
Biotech Company Offers Innovative Tool that has been Frequently Stipulated by Biochemistry Laboratories
ChromoTek, a German biotech company specializing in innovative tools for biochemistry and life cell imaging, has launched the new Myc-Trap® for efficient and ready to use immunoprecipitation of Myc-tagged proteins and their interacting factors. Unlike other immunoprecipitation tools that are based on traditional antibodies, the unique Myc-Trap® needs just one single polypeptide chain to capture the antigen. Therefore the eluted protein fraction is free of heavy and light antibody chain impurities. To request a free sample visit http://resources.chromotek.com/myc-trap.
Of the Myc-Trap’s® launch, CEO Marion Jung said, “The new Myc-Trap® is the most recent addition to a series of innovative Nano-Traps; the product line started with the well-established and superior GFP-Trap® that has been cited in more than 550 peer reviewed publications so far. We intend to develop more Nano-Traps fulfilling the steady demand of our customers. Stay tuned!”
The Myc-Trap® recognizes the Myc-tag at the N-terminus, C-terminus, or at internal sites of the recombinant fusion protein. For gentle elution of bound protein from the Myc-Trap® the corresponding Myc peptide can be used. Derived from the human c-myc oncogene the Myc-tag is a widespread, common polypeptide tag for detecting recombinant protein expression in various cell systems.
ChromoTek offers additional Nano-Traps: GFP-Trap®, RFP-Trap and GST-Trap. For more information about Myc-Trap® please visit: www.chromotek.com