ChromoBlog

Klaus Herick

Recent Posts

GFP-Booster and RFP-Booster conjugated to Alexa Fluor® 647 or conjugated to ATTO647N? That is the question!

Posted by Klaus Herick on Jul 19, 2019 10:12:05 AM

Immunostaining in HeLa cells low expressing Tubulin-GFP.
Left: GFP signal of Tubulin-GFP (green) and DAPI stain (blue);
Right: Tubulin-GFP detection by GFP-Booster coupled to Alexa Fluor 647

We currently offer our GFP- and RFP-Booster conjugated to two different far-red dyes:

Alexa Fluor® 647 and  ATTO647N.

Read More

Topics: GFP-Booster, RFP-Booster, Nanobody, Alexa Fluor

Why are recombinant Nanobodies/ VHHs beneficial?

Posted by Klaus Herick on Jun 13, 2019 4:00:00 PM

Nanobodies are the binding domains of heavy chain only antibodies from Camelids. Nanobodies can be recombinantly produced in bacterial and other animal-free expression systems depending on the actual Nanobody construct. In contrast, classical IgG antibodies are composed of two heavy chains and two light chains and are traditionally produced using hybridoma technlogies or are isolated from a host’s blood.

Read More

Topics: Nanobody, Secondary antibody, Validation, recombinat expression

Advantages of recombinant Nano-Secondaries

Posted by Klaus Herick on May 23, 2019 4:10:12 PM

Nano-Secondaries are monoclonal Nanobodies that bind to primary antibodies in a species and subtype specific manner, i.e. Nano-Secondaries are very precise secondary antibodies. They are recombinantly produced in bacterial or other animal-free expression systems; in contrast, classical mono- or polyclonal secondary antibodies are traditionally produced using hybridoma technologies or are isolated from a host’s blood.

Read More

Topics: Secondary antibody, Validation, recombinat expression, Nano-Secondaries

22 References about Myc-Trap

Posted by Klaus Herick on Sep 6, 2018 4:29:17 PM

We are happy to present an updated list of scientific publications that have been published based on data generated by Myc-Trap. See detailed list below.

Read More

Topics: Nano-Traps, Myc-tag IP, Myc Nanobody, Myc-Trap

First Peptide-tag specific Nanobody applied in super resolution microscopy (SRM)

Posted by Klaus Herick on Mar 2, 2018 11:02:00 AM

You may have noticed the recent publication “A peptide tag-specific nanobody enables high-quality labeling for STORM imaging” of Virant et al (2018) in Nature Communications doi: 10.1038/s41467-018-03191-2, where for the first time a peptide-tag specific Nanobody was applied in dSTORM imaging: The authors have described and discussed the performance of a BC2 peptide-tag specific Nanobody to image BC2 peptide tag fusion proteins in super resolution microscopy, i.e. dSTORM. This Blog features that BC2 peptide-tag specific Nanobody, which ChromoTek markets under the names Spot-Label® and Spot-Trap®. In addition, this Blog figures the significantly improved version of the BC2 peptide -tag, which is called Spot-Tag®.

Read More

Topics: Nanobody, Spot-Tag, Capture/detection System, Spot Nanobody, Spot-Trap, Spot-Label, Spot super resolution microscopy, Spot STED, Spot immunofluorescence, BC2tag

Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins

Posted by Klaus Herick on Jul 6, 2017 1:30:48 PM

Multiple literature references for GST-Trap


This article provides an overview of scientific publications that reference the ChromoTek GST-Trap superior binding performance for various applications. These include immunoprecipitation (IP), Co-IP of Glutathione S-Transferase (GST)-fusion proteins, and Luminex bead assays of GST-tagged and GFP-tagged proteins. Besides, usages for depletion of GST (glutathione sepharose)  after cleavage from recombinant fusion proteins and affinity purification of GST-tagged proteins are possible.

Read More

Topics: Immunoprecipitation, GST, affinity resin, GST immunoprecipitation, references, affinity purification, GST pulldown

ChromoTek Nano-Booster for super resolution microscopy is featured at Biocompare

Posted by Klaus Herick on Jun 2, 2017 4:21:49 PM

ChromoTek’s Marketing Manager Dr. Christoph Eckert has been interviewed by Biocompare, the buyers’ guide for life scientists, on labels for super resolution microscopy.

Read More

Topics: GFP-Booster, RFP-Booster, Super resolution microscopy, VHH, Nanobody

Myc-Trap for both immunoprecipitation and affinity purification of Myc-tagged proteins

Posted by Klaus Herick on Jun 2, 2017 11:41:33 AM

Practical considerations and instructions

Introduction

The ChromoTek Myc-Trap is an affinity resin that consists of Myc-binding protein derived from an Alpaca single domain antibody, also called anti-Myc VHH or “anti-Myc nanobody”, which is coupled to agarose and magnetic agarose beads. The Myc-Trap provides advantages over conventional IgG antibodies when applied in immunoprecipitation (IP) and affinity purification experiments:

Read More

Topics: Immunoprecipitation, Myc-tag, Myc-tag IP, single domain antibody, Myc Nanobody

New limit in protein complex stability: GFP-binding protein:GFP complex

Posted by Klaus Herick on Feb 3, 2017 3:56:34 PM

Although traditional IgG antibodies are often used for immunoprecipitation and protein interaction analysis, GFP-binding protein (GBP, ChromoTek gt-250) and other ChromoTek VHHs (https://www.chromotek.com/about-us/the-alpaca-antibody-advantage/) easily outcompete those under challenging conditions such as elevated temperatures, varying pH or high denaturant concentrations. In fact, GBP tightly binds GFP-fusion proteins even in 8 M urea at 51 °C, which sets a new limit in protein complex stability, virtually unrivalled by any other capture molecule protein tag pair.

Read More

Topics: GFP-Trap, Nano-Trap, GFP, GFP- Binding Protein

ChromoTek’s camelid antibodies are sequenced, recombinant, and monoclonal with validated binding characteristics

Posted by Klaus Herick on Jul 25, 2016 2:35:54 PM

On a personal note, we – ChromoTek - like to state a few words on the quality of our camelid VHH or heavy chain antibodies (also known as “nanobodies”).

Read More

Topics: Antibody Quality

News about Nanobodies

For researchers interested in

  • New Nanobody based tools
  • Tips and tricks
  • Featured publications

Subscribe Here!

Recent Posts

Posts by Tag

See all