ChromoBlog

Klaus Herick

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22 References about Myc-Trap

Posted by Klaus Herick on Sep 6, 2018 4:29:17 PM

We are happy to present an updated list of scientific publications that have been published based on data generated by Myc-Trap. See detailed list below.

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Topics: Nano-Traps, Myc Nanobody, Myc-tag IP, Myc-Trap

First Peptide-tag specific Nanobody applied in super resolution microscopy (SRM)

Posted by Klaus Herick on Mar 2, 2018 11:02:00 AM

You may have noticed the recent publication “A peptide tag-specific nanobody enables high-quality labeling for STORM imaging” of Virant et al (2018) in Nature Communications doi: 10.1038/s41467-018-03191-2, where for the first time a peptide-tag specific Nanobody was applied in dSTORM imaging: The authors have described and discussed the performance of a BC2 peptide-tag specific Nanobody to image BC2 peptide tag fusion proteins in super resolution microscopy, i.e. dSTORM. This Blog features that BC2 peptide-tag specific Nanobody, which ChromoTek markets under the names Spot-Label® and Spot-Trap®. In addition, this Blog figures the significantly improved version of the BC2 peptide -tag, which is called Spot-Tag®.

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Topics: Spot-Tag, Spot-Trap, Spot-Label, Capture/detection System, Spot Nanobody, Nanobody, Spot immunofluorescence, Spot super resolution microscopy, Spot STED, BC2tag

Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins

Posted by Klaus Herick on Jul 6, 2017 1:30:48 PM

Multiple literature references for GST-Trap


This article provides an overview of scientific publications that reference the ChromoTek GST-Trap superior binding performance for various applications. These include immunoprecipitation (IP), Co-IP of Glutathione S-Transferase (GST)-fusion proteins, and Luminex bead assays of GST-tagged and GFP-tagged proteins. Besides, usages for depletion of GST (glutathione sepharose)  after cleavage from recombinant fusion proteins and affinity purification of GST-tagged proteins are possible.

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Topics: GST, Immunoprecipitation, affinity purification, affinity resin, GST pulldown, GST immunoprecipitation, references

ChromoTek Nano-Booster for super resolution microscopy is featured at Biocompare

Posted by Klaus Herick on Jun 2, 2017 4:21:49 PM

ChromoTek’s Marketing Manager Dr. Christoph Eckert has been interviewed by Biocompare, the buyers’ guide for life scientists, on labels for super resolution microscopy.

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Topics: GFP-Booster, RFP-Booster, Super resolution microscopy, VHH, Nanobody

Myc-Trap for both immunoprecipitation and affinity purification of Myc-tagged proteins

Posted by Klaus Herick on Jun 2, 2017 11:41:33 AM

Practical considerations and instructions

Introduction

The ChromoTek Myc-Trap is an affinity resin that consists of Myc-binding protein derived from an Alpaca single domain antibody, also called anti-Myc VHH or “anti-Myc nanobody”, which is coupled to agarose and magnetic agarose beads. The Myc-Trap provides advantages over conventional IgG antibodies when applied in immunoprecipitation (IP) and affinity purification experiments:

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Topics: Immunoprecipitation, Myc-tag IP, Myc-tag, single domain antibody, Myc Nanobody

New limit in protein complex stability: GFP-binding protein:GFP complex

Posted by Klaus Herick on Feb 3, 2017 3:56:34 PM

Although traditional IgG antibodies are often used for immunoprecipitation and protein interaction analysis, GFP-binding protein (GBP, ChromoTek gt-250) and other ChromoTek VHHs (https://www.chromotek.com/about-us/the-alpaca-antibody-advantage/) easily outcompete those under challenging conditions such as elevated temperatures, varying pH or high denaturant concentrations. In fact, GBP tightly binds GFP-fusion proteins even in 8 M urea at 51 °C, which sets a new limit in protein complex stability, virtually unrivalled by any other capture molecule protein tag pair.

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Topics: GFP-Trap, Nano-Trap, GFP- Binding Protein, GFP

ChromoTek’s camelid antibodies are sequenced, recombinant, and monoclonal with validated binding characteristics

Posted by Klaus Herick on Jul 25, 2016 2:35:54 PM

On a personal note, we – ChromoTek - like to state a few words on the quality of our camelid VHH or heavy chain antibodies (also known as “nanobodies”).

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Topics: Antibody Quality

ChromoTek on tour in Germany!

Posted by Klaus Herick on Oct 21, 2015 12:08:22 PM

ChromoTek meets researchers at their institutes in Germany: From October 27 to 30, 2015 our senior scientists Dr. Andrea Buchfellner and Dr. Larisa Yurlova will be on a 2.000 km road show and provide scientific seminar lecture series. They will deliver presentations on two topics as requested by local scientists:

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ChromoTek GmbH has Launched the New Myc-Trap® for Immunoprecipitation of Myc-Tagged Proteins

Posted by Klaus Herick on Jul 10, 2015 2:44:37 PM

Biotech Company Offers Innovative Tool that has been Frequently Stipulated by Biochemistry Laboratories

ChromoTek, a German biotech company specializing in innovative tools for biochemistry and life cell imaging, has launched the new Myc-Trap® for efficient and ready to use immunoprecipitation of Myc-tagged proteins and their interacting factors. Unlike other immunoprecipitation tools that are based on traditional antibodies, the unique Myc-Trap® needs just one single polypeptide chain to capture the antigen. Therefore the eluted protein fraction is free of heavy and light antibody chain impurities. To request a free sample visit http://resources.chromotek.com/myc-trap.

Of the Myc-Trap’s® launch, CEO Marion Jung said, “The new Myc-Trap® is the most recent addition to a series of innovative Nano-Traps; the product line started with the well-established and superior GFP-Trap® that has been cited in more than 550 peer reviewed publications so far. We intend to develop more Nano-Traps fulfilling the steady demand of our customers. Stay tuned!

The Myc-Trap® recognizes the Myc-tag at the N-terminus, C-terminus, or at internal sites of the recombinant fusion protein. For gentle elution of bound protein from the Myc-Trap® the corresponding Myc peptide can be used. Derived from the human c-myc oncogene the Myc-tag is a widespread, common polypeptide tag for detecting recombinant protein expression in various cell systems.

ChromoTek offers additional Nano-Traps: GFP-Trap®, RFP-Trap and GST-Trap. For more information about Myc-Trap® please visit: www.chromotek.com

 

Request free Myc-Trap sample

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