ChromoTek’s Marketing Manager Dr. Christoph Eckert has been interviewed by Biocompare, the buyers’ guide for life scientists, on labels for super resolution microscopy.
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Topics: GFP-Booster, RFP-Booster, Super resolution microscopy, VHH, Nanobody
Practical considerations and instructions
Introduction
The ChromoTek Myc-Trap is an affinity resin that consists of Myc-binding protein derived from an Alpaca single domain antibody, also called anti-Myc VHH or “anti-Myc nanobody”, which is coupled to agarose and magnetic agarose beads. The Myc-Trap provides advantages over conventional IgG antibodies when applied in immunoprecipitation (IP) and affinity purification experiments:
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Topics: Immunoprecipitation, Myc-tag, Myc-tag IP, single domain antibody, Myc Nanobody
Although traditional IgG antibodies are often used for immunoprecipitation and protein interaction analysis, GFP-binding protein (GBP, ChromoTek gt-250) and other ChromoTek VHHs (https://www.chromotek.com/about-us/the-alpaca-antibody-advantage/) easily outcompete those under challenging conditions such as elevated temperatures, varying pH or high denaturant concentrations. In fact, GBP tightly binds GFP-fusion proteins even in 8 M urea at 51 °C, which sets a new limit in protein complex stability, virtually unrivalled by any other capture molecule protein tag pair.
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Topics: GFP-Trap, Nano-Trap, GFP, GFP- Binding Protein
On a personal note, we – ChromoTek - like to state a few words on the quality of our camelid VHH or heavy chain antibodies (also known as “nanobodies”).
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Topics: Antibody Quality
ChromoTek meets researchers at their institutes in Germany: From October 27 to 30, 2015 our senior scientists Dr. Andrea Buchfellner and Dr. Larisa Yurlova will be on a 2.000 km road show and provide scientific seminar lecture series. They will deliver presentations on two topics as requested by local scientists:
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Biotech Company Offers Innovative Tool that has been Frequently Stipulated by Biochemistry Laboratories
ChromoTek, a German biotech company specializing in innovative tools for biochemistry and life cell imaging, has launched the new Myc-Trap® for efficient and ready to use immunoprecipitation of Myc-tagged proteins and their interacting factors. Unlike other immunoprecipitation tools that are based on traditional antibodies, the unique Myc-Trap® needs just one single polypeptide chain to capture the antigen. Therefore the eluted protein fraction is free of heavy and light antibody chain impurities. To request a free sample visit http://resources.chromotek.com/myc-trap.
Of the Myc-Trap’s® launch, CEO Marion Jung said, “The new Myc-Trap® is the most recent addition to a series of innovative Nano-Traps; the product line started with the well-established and superior GFP-Trap® that has been cited in more than 550 peer reviewed publications so far. We intend to develop more Nano-Traps fulfilling the steady demand of our customers. Stay tuned!”
The Myc-Trap® recognizes the Myc-tag at the N-terminus, C-terminus, or at internal sites of the recombinant fusion protein. For gentle elution of bound protein from the Myc-Trap® the corresponding Myc peptide can be used. Derived from the human c-myc oncogene the Myc-tag is a widespread, common polypeptide tag for detecting recombinant protein expression in various cell systems.
ChromoTek offers additional Nano-Traps: GFP-Trap®, RFP-Trap and GST-Trap. For more information about Myc-Trap® please visit: www.chromotek.com
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