Cell cycle and cell proliferation are tightly controlled cellular processes, and their deregulation is a hallmark of cancer. To enable non-invasive analysis of cell cycle in living cells, ChromoTek developed a Cell Cycle Chromobody. The Cell Cycle Chromobody (CCC) consists of a binding domain of a heavy-chain antibody highly specific against human proliferating cell nuclear antigen (PCNA), which is genetically fused to a fluorescent protein.
The ChromoTek Actin Chromobody is a live-cell probe for visualization of the actin cytoskeleton and monitoring its dynamics. The Actin Chromobody enables non-invasive labeling of actin microfilaments not only in mammalian cells, but also in cells and tissues of evolutionary distant species, such as Zebrafish (Panza et al. 2015) or plants (Rocchetti, Hawes, and Kriechbaumer 2014). The Z-stack shows confocal images of optical sections of Actin Chromobody in a Hela cell.
ChromoTek Nano-Boosters are ideal for Super-Resolution and “traditional” fluorescence microscopy because of their high affinity and extremely small size of just 2 to 3 nm. Technically speaking, the GFP-and RFP-Boosters are composed of the highly specific GFP- or RFP-binding domains of alpaca antibodies (also called “nanobodies”), covalently coupled to a selection of fluorescent dyes.
Chromotek’s tiniest GFP-binding alpaca antibodies (also termed nanobodies) are now available conjugated to the Abberior STAR dyes. We developed these new GFP-Boosters to achieve the highest resolution and the best signal in STED imaging of GFP-fusion proteins. The new Boosters are the combination of:
- Chromotek’s anti-GFP alpaca antibody fragments, which are characterized by the nanomolar affinity to GFP and extraordinarily small size (13 kDa, 2 X 3 nm)
Today, Eric Betzig, Stefan W Hell and William E Moerner were awarded the Nobel Prize in Chemistry for surpassing the limitations of light microscopy (www.nobelprize.org/nobel_prizes/chemistry/laureates/2014/press.html). ChromoTek cordially congratulates the laureates of this most prestigious science award. We are especially happy for our prominent customer, friend and endorser Stefan Hell! Stefan pioneered the nanoscopy world and we are delighted that his work and the stimulated emission depletion (STED) microscopy method developed by him have now received this high recognition.
ChromoTek GmbH (Martinsried, Germany) announces the launch of a new research assay for protein-protein interaction (PPI)analysis in live mammalian cells, the Fluorescence Two-Hybrid (F2H®) Kit. This new product is the result of Chromotek's long experience with applying the F2H technology in PPI compound screening for pharmaceutical and biotech customers. The new kit format overcomes limitations of currently available methods and will fascinate biomedical scientists, who investigate protein-protein interactions (PPIs) by its ease of use.
ChromoTek’s F2H® Kit enables effortless analysis of interactions between any GFP- and RFP- tagged protein pairs in living mammalian cells by conventional fluorescence microscopy. It is a fast, simple and quantitative way to characterize PPIs in intracellular environment, screen for PPI inhibitors and evaluate their activity in real time. Unlike available complementation assays, such as split-YFP, F2H® is a fully reversible assay and therefore better suitable for testing PPI antagonists. In comparison to FRET assays, the ingeniously simple optical read-out of F2H® does not require sophisticated equipment, making it much more affordable and at the same time robust and reliable.
Yes! Our latest paper reporting on screening for protein-protein interaction inhibitors with F2H is now online: http://www.ncbi.nlm.nih.gov/pubmed/24476585.