ChromoTek’s Marketing Manager Dr. Christoph Eckert has been interviewed by Biocompare, the buyers’ guide for life scientists, on labels for super resolution microscopy.
See the interview here:
Christoph outlines the dedicated advantages of ChromoTek’s Nano-Boosters, which base on small size and superior binding:
- Less than 2nm epitope-label displacement minimizes linkage error
- Better tissue penetration
- Higher image resolution
- Stabilization, enhancement and reactivation of GFP and RFP signals after cell fixation, chemical, and thermal treatments
- No clustering because nanobodies are monovalent
Relative size comparison of Nano-Boosters and complex of conventional primary and secondary antibody complex (IgG complex). Due to their small size, Nano-Boosters penetrate tissue better. In addition, they bind closer to their targets than conventional antibodies. This results in better staining and higher resolution.
ChromoTek Nano-Boosters bind to the following targets:
mRFP, mCherry, mRFPruby, mPlum
eGFP, wtGFP, GFP S65T, AcGFP, TagGFP, tagGFP2, sfGFP, pHluorin, eYFP, YFP, Venus, Citrine, eCFP, CFP, mCerulean
ChromoTek Nano-Boosters are derived from single-domain alpaca antibody fragments, termed VHHs or “nanobodies”. Nanobodies conjugated to fluorescent dyes are known as Nano-Boosters.
ChromoTek is the pioneer of protein-protein interactions, who has brought fluorescent proteins from cell biology as protein tags into biochemistry laboratories. Today, particularly the ChromoTek GFP-Trap is frequently used for additional applications beyond pulldowns, because of its outstanding binding of GFP-fusions.