Immunostaining in HeLa cells low expressing Tubulin-GFP.
Left: GFP signal of Tubulin-GFP (green) and DAPI stain (blue);
Right: Tubulin-GFP detection by GFP-Booster coupled to Alexa Fluor 647
We currently offer our GFP- and RFP-Booster conjugated to two different far-red dyes:
Alexa Fluor® 647 and ATTO647N.
The GFP- Booster Alexa Fluor® 647 and RFP-Booster Alexa Fluor® 647 have multiple advantages when compared to GFP-/ RFP-Booster ATTO647N:
Improved GFP/RFP-Booster |
GFP/RFP-Booster ATTO647N |
Brighter fluorescence signal |
About one third as bright |
Site directed labelled |
Labelled at C-terminus |
Degree of labeling DOL = 2 dyes per VHH |
Labelled with average |
Dilution range: 1:500 – 1:1,000 |
Dilution: 1:200 |
Order information |
Order information |
Please note that we intend to replace GFP/-RFP-Booster ATTO 647N by GFP-/ RFP-Booster Alexa Fluor 647.
Please contact us if you require an ongoing supply of GFP-Booster or RFP-Booster conjugated to ATTO 647N. We are curious to learn why you need GFP/ RFP-Booster ATTO647N and why you can’t use GFP/RFP-Booster Alexa Fluor 647 instead. Email to support@chromotek.com