Chromotek’s tiniest GFP-binding alpaca antibodies (also termed nanobodies) are now available conjugated to the Abberior STAR dyes. We developed these new GFP-Boosters to achieve the highest resolution and the best signal in STED imaging of GFP-fusion proteins. The new Boosters are the combination of:
- Chromotek’s anti-GFP alpaca antibody fragments, which are characterized by the nanomolar affinity to GFP and extraordinarily small size (13 kDa, 2 X 3 nm)
and
- STED-optimized far-red dyes from Abberior: Abberior STAR RED or Abberior STAR 635P.
Image: Vimentin-GFP immunostained with GFP-Booster-STAR635P in confocal and STED. Abberior STAR 635P dye was provided by Abberior http://www.abberior.com/products/productlist/cat/sted-resolft/prod/abberior-star-635p/, the STED image was obtained with Abberior Instruments STED Microscope http://abberior-instruments.net/home/. (Images are courtesy of G. Donnert and C. Wurm, Abberior GmbH.)
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