ChromoBlog

Chromotek’s GFP- binding protein, an anti- GFP Nanobody, is a very small and effective tool for binding and visualizing GFP-tagged proteins. In their recent publication, Modi et al. successfully functionalized quantum dots with the GFP- Nanobody (QD GFP- Nanobody). Thus, they created a small GFP- specific label with a very strong fluorescent signal.

QD-Nanobody ChromoTek

In “Quantum dot conjugated nanobodies for multiplex imaging of protein dynamics at synapses“, Modi et al. successfully used these QD Nanobodies for single particle tracking.

They showed that

  • ChromoTek’s GFP binding protein (GFP nanobody) can be coupled to quantum dots
  • QD nanobodies can be used for single particle tracking of membrane proteins irrespective of cell type
  • QD-nanobody conjugates improve accessibility to more complex diffusion environments such as synaptic sites or intact tissues where accessibility and penetration are challenging
  • Multiplexing with differently colored Quantum dots and different Nanobodies (QD GFP-Nanobody and QD RFP-Nanobody) is possible.

Nanobodies, also termed VHHs, are very small (15kDa) and stable binding proteins derived from single domain antibody fragments. ChromoTek offers GFP- Nanobodies and RFP- Nanobodies.

Quantum dots are very bright fluorescent nanocrystals with high photostability.

Reference:
Modi S, Higgs NF, Sheehan D ,Griffin LD ,Kittler JT
Quantum dot conjugated nanobodies for multiplex imaging of protein dynamics at synapses.
Nanoscale. 2018 May 31;10(21):10241-10249. doi: 10.1039/c7nr09130c.


Topics: GFP Nanobody, RFP Nanobody, Quantum dots, single particle tracking, Quantum dot Nanobody

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