ChromoBlog

Working with green fluorescent proteins: Tools and properties

Posted by Christoph Eckert on Feb 7, 2019 1:18:14 PM

Jellyfish Green Fluorescent Protein (GFP) and its derivatives are still the most frequently used fluorescent proteins in biomedical research. Recently, additional green fluorescent proteins have been discovered in higher animals such as crustaceans and lancelets. These FPs share a common fold, but diverge widely in their primary sequence. Thus, they require novel, dedicated antibody research tools. Here is an overview about EGFP (the most commonly used GFP derivative), TurboGFP and mNeonGreen.

 

EGFP
(enhanced GFP)

TurboGFP

mNeonGreen

Discovery/
first publication

1995

2004

2013

Structure

GFP

turboGFP

mNeonGreen

Origin

GFP from Jellyfish
Aequorea victoria

Tetrameric CopGFP from copepod Pontellina plumata

Tetrameric LanYFP from Lancelet Branchiostoma lanceolatum

Sequence identity to EGFP

(100%)

~20%

20-25%

Derived from GFP?

Yes

No

No

Structure

Monomer

Dimer

Monomer

Length/ molecular weight (MW)

239 amino acids,
26.9 kDa

2x 232 amino acids,
2x 26 kDa

237 amino acids,
26.6 kDa

Excitation / Emission maximum

488/ 509 nm

482 nm/ 502 nm

506 nm/ 517 nm

Common variants

GFP, CFP, YFP, Venus, BFP and many more

TurboGFP, CopGFP, max GFP, maxFP-Green

mNeonGreen

Research Tools

Immunoprecipitation
GFP-Trap

Immunofluorescence
GFP-Booster,
GFP antibody PABG1

Western Blotting

GFP antibody [3H9]
GFP antibody [PABG1]

Control
purified EGFP protein

Immunoprecipitation
TurboGFP-Trap

Immunoprecipitation
mNeonGreen-Trap

Immunofluorescence
mNeonGreen antibody [32F6]

Western Blotting
mNeonGreen antibody [32F6]

 


Working with GFP, TurboGFP or mNeonGreen?

Doing immunoprecipitations?

Request test sample

 

Topics: Nanobody, mNeonGreen, mNeonGreen immunoprecipitation, GFP, GFP Immunoprecipitation, TurboGFP, TurboGFP immunoprecipitation, EGFP

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