ChromoBlog

22 References about Myc-Trap

Posted by Klaus Herick on Sep 6, 2018 4:29:17 PM

We are happy to present an updated list of scientific publications that have been published based on data generated by Myc-Trap. See detailed list below.

ChromoTek Myc-Trap® is an established tool for csm_Myc-Trap_A_Grafik_Gel_e8a1404eeaimmunoprecipitations (IPs), Co-IPs and affinity purifications of Myc-tagged proteins. It is based on an anti-Myc Nanobody derived from alpaca coupled to a matrix.

As you can see in below list, applications comprise

  • Immunoprecipitation (IP)
  • Co-IP
  • Co-IP for Mass Spec (also called AP/MS).

Proteins from multiple organisms or cells have been pulled down. Organisms and cells include:

  • mammalian cell lines: HeLa, HEK293T, CHO
  • drosophila melanogaster
  • Danio rerio (zebrafish)
  • toxoplasma gondii
  • nicotiana benthamiana
  • arabidopsis thaliana

 

2018

1
Alternative splicing of (ppp1r12a/mypt1) in zebrafish produces a novel myosin phosphatase targeting subunit
Andrew LaFlamme, Kyle E.Young, IreneLang, and Douglas C.Weiser
Gene (2018) 675: 15-26 - https://doi.org/10.1016/j.gene.2018.06.092
Products and applications: Myc-Trap, IP

2
Proteomic and Biochemical Comparison of the Cellular Interaction Partners of Human VPS33A and VPS33B
Morag R.Hunter, Geoffrey G.Hesketh, Tomasz H.Benedyk, Anne-ClaudeGingras, and Stephen C.Graham
Journal of Mol Biology (2018) 430, 14: 2153-2163 - https://doi.org/10.1016/j.jmb.2018.05.019
Products and applications: Myc-Trap, GFP-Trap, Co-IP

3
UCHL3 Regulates Topoisomerase-Induced Chromosomal Break Repair by Controlling TDP1 Proteostasis
Chunyan Liao, Ryan Beveridge, Jessica J.R. Hudson, Jacob D. Parker, Shih-Chieh Chiang, Swagat Ray, Mohamed E. Ashour, Ian Sudbery, Mark J. Dickman, Sherif F. El-Khamisy
Cell Reports (2018) 23, 11: 3352-3365 - https://doi.org/10.1016/j.celrep.2018.05.033
Products and applications: GFP-Trap, Myc-Trap, Co-IP

4
Two essential Thioredoxins mediate apicoplast biogenesis, protein import, and gene expression in Toxoplasma gondii
Marco Biddau, Anne Bouchut, Jack Major, Tracy Saveria, Julie Tottey, Ojore Oka, Marcel van-Lith, Katherine Elizabeth Jennings, Jana Ovciarikova, Amy DeRocher, Boris Striepen, Ross Frederick Waller, Marilyn Parsons, Lilach Sheiner
PLOS Pathogens (2018) - https://doi.org/10.1371/journal.ppat.1006836
Products and applications: Myc-Trap, Co-IP/MS (AP/MS)

5
RIT1 controls actin dynamics via complex formation with RAC1/CDC42 and PAK1
Uta Meyer zum Büschenfelde, Laura Isabel Brandenstein, Leonie von Elsner, Kristina Flato, Tess Holling, Martin Zenker, Georg Rosenberger, Kerstin Kutsche
PLOS Genetics (2018) - https://doi.org/10.1371/journal.pgen.1007370
Products and applications: Myc-Trap, Co-IP

6
Human-Specific NOTCH2NL Genes Expand Cortical Neurogenesis through Delta/Notch Regulation
Ikuo K. Suzuki, David Gacquer, Roxane Van Heurck, Devesh Kumar, Marta Wojno, Angéline Bilheu, Adèle Herpoel, Nelle Lambert, Julian Cheron, Franck Polleux, Vincent Detours, Pierre Vanderhaeghen
Cell (2018) 173, 6: 1370-1384 - https://doi.org/10.1016/j.cell.2018.03.067
Products and applications: Myc-Trap, GFP-Trap; Co-IP

7
Vaccinia virus C9 ankyrin-repeat F-box protein is a newly identified antagonist of the type I interferon-induced antiviral state
Ruikang Liu and Bernard Moss
Virol. (2018) - https://doi.org/10.1128/JVI.00053-18
Products and applications: Myc-Trap, IP

8
Toxoplasma gondii chromosomal passenger complex is essential for the organization of a functional mitotic spindle: a prerequisite for productive endodyogeny
Laurence Berry, Chun-Ti Chen, Maria E. Francia, Amandine Guerin, Arnault Graindorge, Jean-Michel Saliou, Maurane Grandmougin, Sharon Wein, Chérine Bechara, Juliette Morlon-Guyot, Yann Bordat, Marc-Jan Gubbels, Maryse Lebrun, Jean-François Dubremetz, Wassim Daher
Cellular and Molecular Life Sciences (2018): 1-27 - doi.org/10.1007/s00018-018-2889-6
Products and applications: Myc-Trap, GFP-Trap, IP

9
Dimer‐specific immunoprecipitation of active caspase‐2 identifies TRAF proteins as novel activators
Alexander C Robeson, Kelly R Lindblom, Jeffrey Wojton, Sally Kornbluth, Kenkyo Matsuura
The EMBO Journal (2018) - https://doi.org/10.15252/embj.201797072
Products and applications: Myc-Trap, GFP-Trap, IP; monoclonal anti-GFP antibody 3H9, Western blot

10
Targeted Recruitment of the Basal Transcriptional Machinery by LNK Clock Components Controls the Circadian Rhythms of Nascent RNAs in Arabidopsis
Yuan Ma, Sergio Gil, Klaus D. Grasser, and Paloma Mas
The Plant Cell (2018) 30: 907–924 - https://doi.org/10.1105/tpc.18.00052
Products and applications: Myc-Trap, GFP-Trap, Co-IP

11
Phosphorylation by CK2 regulates MUS81/EME1 in mitosis and after replication stress
Anita Palma, Giusj Monia Pugliese, Ivana Murfuni, Veronica Marabitti, Eva Malacaria, Sara Rinalducci, Anna Minoprio, Massimo Sanchez, Filomena Mazzei, Lello Zolla, Annapaola Franchitto, Pietro Pichierri
Nucleic Acids Research (2018) 46, 10: 5109–5124 - https://doi.org/10.1093/nar/gky280
Products and applications: Myc-Trap, IP

12
RNF144A Sustains EGFR Signaling to Promote EGF-Dependent Cell Proliferation
Shiuh-Rong Ho and Weei-Chin Lin
JBC (2018) - https://doi.org/10.1074/jbc.RA118.002887
Products and applications: Myc-Trap, IP

13
Presynaptic Biogenesis Requires Axonal Transport of Lysosome-Related Vesicles
Anela Vukoja, Ulises Rey, Astrid G. Petzoldt, Christoph Ott, Dennis Vollweiter, Christine Quentin, Dymtro Puchkov, Eric Reynolds, Martin Lehmann, Svea Hohensee, Stefanie Rosa, Reinhard Lipowsky, Stephan J. Sigrist, VolkerHaucke
Neuron (2018) 99: 1-17 - https://doi.org/10.1016/j.neuron.2018.08.004
Products and applications: Myc-Trap, Co-IP

2017

14
Peroxisomal ACBD4 interacts with VAPB and promotes ER-peroxisome associations
Joseph L. Costello, Inês G. Castro, Tina A. Schrader, Markus Islinger & Michael Schrader
Cell Cycle (2017): 1039-1045 - https://doi.org/10.1080/15384101.2017.1314422
Products and applications: Myc-Trap, GFP-Trap, Co-IP/MS (AP/MS)

15
IFT proteins spatially control the geometry of cleavage furrow ingression and lumen positioning
Nicolas Taulet, Benjamin Vitre, Christelle Anguille, Audrey Douanier, Murielle Rocancourt, Michael Taschner, Esben Lorentzen, Arnaud Echard & Benedicte Delava
Nature Communications (2017) 8: 1928 - https://doi.org/10.1038/s41467-017-01479-3
Products and applications: Myc-Trap, GFP-Trap, Co-IP

16
Selective transport of neurotransmitters and modulators by distinct volume-regulated LRRC8 anion channels
Darius Lutter, Florian Ullrich, Jennifer C. Lueck, Stefan Kempa & Thomas J. Jentsch
JCS (2017) - https://doi.org/10.1242/jcs.196253
Products and applications: Myc-Trap, GFP-Trap, Co-IP

17
The activity of the Drosophila Vestigial protein is modified by Scalloped-dependent phosphorylation
Virginia L.Pimmetta, HuaDengab, Julie A.Haskinsa, Rebecca J.Merciera, PaulLaPointea, Andrew J.Simmondsa
Developmental Biology (2017) 425, 1: 58-69 - https://doi.org/10.1016/j.ydbio.2017.03.013
Products and applications: Myc-Trap, Co-IP

18
Endophilin B2 facilitates endosome maturation in response to growth factor stimulation, autophagy induction, and influenza A virus infection
Jacob M Serfass, Yoshinori Takahashi, Zhixiang Zhou, Yuka Imamura Kawasawa, Ying Liu, Nikolaos Tsotakos, Megan M Young, Zhenyuan Tang, Linlin Yang, Jennifer M. Atkinson, Zissis C Chroneos and Hong-Gang Wang
JBC (2017) - https://doi.org/10.1074/jbc.M117.792747
Products and applications: Myc-Trap, IP

19
The E3 ligase TaSAP5 alters drought stress responses by promoting the degradation of DRIP proteins
Ning Zhang, Yujing Yin, Xinye Liu, Shaoming Tong, Jiewen Xing, Yuan Zhang, Ramesh N. Pudake, Edenys Miranda Izquierdo, Huiru Peng, Mingming Xin, Zhaorong Hu, Zhongfu Ni, Qixin Sun, Yingyin Yao
Plant Physiology (2017) - https://doi.org/10.1104/pp.17.01319
Products and applications: Myc-Trap, GFP-Trap, Co-IP

20
Carbonic Anhydrases Function in Anther Cell Differentiation Downstream of the Receptor-like Kinase EMS1
Jian Huang, Zhiyong Li, Gabriel Biener, Erhui Xiong, Shikha Malik, Nathan Eaton, Catherine Z. Zhao, Valerica Raicu, Hongzhi Kong, and Dazhong Zhao
Plant Cell (2017) - https://doi.org/10.1105/tpc.16.00484
Products and applications: Myc-Trap, Co-IP

 

2016

21
Avr4 promotes Cf-4 receptor-like protein association with the BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and plant immunity
Jelle Postma, Thomas W. H. Liebrand, Guozhi Bi, Alexandre Evrard, Ruby R. Bye, Malick Mbengue, Hannah Kuhn, Matthieu H. A. J. Joosten and Silke Robatzek
New Phytologist (2016) 210: 627–642 - https://doi.org/10.1111/nph.13802
Products and applications: Myc-Trap, Co-IP

22
Two SERK receptor-like kinases interact with the receptor-like kinase EMS1 in Anther Cell Fate Determination
Zhiyong Li, Yao Wang, Jian Huang, Nagib Ahsan, Gabriel Biener, Joel Paprocki, Jay J. Thelen, Valerica Raicu, and Dazhong Zhao
Plant Physiology (2016) - https://doi.org/10.1104/pp.16.01219
Products and applications: Myc-Trap, Co-IP

 

Tips & Tricks and Test Sample:

When you plan the IP, Co-IP, and/or affinity purification of your Myc-tagged protein, you may want to consider using 1xMyc-tagged proteins ore 2x Myc-tagged proteins. Depending on your needs, you may further improve your experiment:

1xMyc-tagged protein

2xMyc-tagged protein

Ideal for gentle and effective elution of native protein

Ideal for low expressed proteins

Extraordinary strong binding


More details about experimental setup, epitope, binding affinities and elution strategies?

Download whitepaper


You want to test the Myc-Trap for yourself?

Request test sample

 

Topics: Nano-Traps, Myc-tag IP, Myc Nanobody, Myc-Trap

Subscribe to Email Updates

Recent Posts

Posts by Topic

see all