ChromoBlog

Unique Vimentin probes presented at AACR 2017 annual meeting

Posted by Christoph Eckert on Mar 28, 2017 10:00:00 AM

Vimentin dynamics can be effectively visualized using the ChromoTek Vimentin-Chromobody, e.g. to monitor epithelial-mesenchymal transition (EMT). To learn more about this unique probe attend Julia Maier’s talk “Tracing EMT with fluorescent biosensors (Chromobodies) in living lung cancer cells” on April 3, 4:20 pm in Room 144, Level 1 at AACR 2017 in Washington, DC.

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Topics: Chromobodies, Chromobody

Actin Chromobody: Visualization of actin dynamics – NOT alteration

Posted by Christoph Eckert on Feb 20, 2017 1:21:56 PM

Everybody really wants to use a detection probe that does not influence its target. For measurement of actin and actin-related functions there are a number of actin visualization probes and techniques available that researchers can select. Hence, one should only apply such actin visualization probes, which do not alter actin dynamics. How do the actin probes for visualization perform? Which probes do interfere with function and which probes do not? Robert Grosse and his team from the Institute of Pharmacology at the Biochemical-Pharmacological Center (BPC), University of Marburg, Germany took the effort and systematically looked into detection technologies of actin filaments in order to avoid potential pitfalls. In the recently published mini review/poster “Actin Visualization at a Glance” (Melak et al. 2017) they have discussed the pros and cons of current probes to visualize actin filaments, i.e. Phalloidin, LifeAct, Utrophin, F-tractin, SiR-actin, GFP-actin, tagged actin, and Actin Chromobody® (see table 1 and figure 1 below). 

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Topics: Chromobodies, Actin Chromobody, cytoskeleton, Actin, live cell imaging

Visualize the onset of metastasis with Chromobodies

Posted by Christoph Eckert on Oct 4, 2016 4:00:00 PM

The progression of epithelial cancers into metastatic stages is triggered by a cellular process called “epithelial-mesenchymal transition” (EMT). In response to cytokines, tumor cells shut down the expression of epithelial makers including occludin or E-cadherin and gain mesenchymal markers such as N-cadherin or vimentin. Such cells are losing their cell-cell contacts and apical-basal polarity and become highly migratory and invasive. Considering the importance of this process for cancer progression and formation of metastasis, reliable cellular EMT biomarker and versatile detection systems are necessary to develop novel anti-metastatic therapies.

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Topics: Chromobodies

Chromobody® plasmids: Good things come to those who wait!

Posted by Kourosh Zolghadr on Jun 5, 2014 4:50:00 PM

This time last spring we started marketing our Chromobody® plasmids. Our customers realized the impact of the technology and were immediately beginning to work with the Chromobodies®. Now, one year later the first papers are being published demonstrating the broad applicability of this intracellular fluorescent antibody technology: 

The first is a paper in Molecular Cell by the Lindqvist Lab at the Karolinska Institutet in Stockholm, Sweden. They developed a FRET-based system for accurate quantification of fluorescence from single cells. By visualizing endogenous PCNA at replication foci with the Cell Cycle Chromobody® they nicely show that the mitotic entry network is linked to the completion of S phase. It does not depend on protein accumulation through G2 but is activated by mitotic phosphorylations at the end of S phase. The method they present allows analyzing live-cell as well as extracting temporal information from fixed cells based on endogenous marker proteins. (Akopyan, K.; Silva Cascales, H.; Hukasova, E., et al. Assessing kinetics from fixed cells reveals activation of the mitotic entry network at the S/G2 transition. Molecular cell. 2014, 53, 843-853. http://dx.doi.org/10.1016/j.molcel.2014.01.031

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Topics: Chromobodies, Cell Cycle Chromobody, Chromobody, Chromobody plasmid, Actin Chromobody, Lamin Chromobody, Dnmt1 Chromobody, custom Chromobody, intracellular antibody, PCNA

ChromoTek and NMI TT ally to start a new chapter in HCA

Posted by Katrin Schmidthals on Mar 24, 2014 6:01:00 PM

ChromoTek GmbH (Martinsried, Germany) and NMI Technology Transfer GmbH (Reutlingen, Germany) have joined forces to advance ChromoTek ́s proprietary Chromobody® technology in new cellular models. 

Chromobodies® are new intracellular functional antibodies visualising dynamic changes of target proteins in live cells. With the help of NMI`s outstanding lentiviral transduction technology, Chromobodies® will be introduced into difficult-to-transfect cell lines such as primary cells. The cooperation will establish innovative cell lines available for phenotypic screening, target validation and high content analysis (HCA). The unique combination of Chromobody® nanoprobes and a comprehensive collection of disease-relevant cellular test systems will lift preclinical compound screening and pathway analysis in the early drug discovery process to a new level of performance.

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Topics: Chromobodies, Nano-Traps, Nano-Booster, F2H assay, GFP-Trap, Nano-Trap, high content analysis, HCA, Chromobody

Meet us at #SfN13

Posted by Joerg Katzenberger on Nov 9, 2013 8:45:00 PM

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Topics: Chromobodies, Nano-Traps, Nano-Booster, F2H assay, GFP-Trap, Nano-Trap, Roadshow, USA, protein-protein interaction

We present two posters at #MipTec2013 in Basel

Posted by Joerg Katzenberger on Sep 23, 2013 9:56:00 PM
 
  • Intracellular Fluorescent Alpaca Antibodies (Chromobodies):
    Non-invasive Biomarkers for High-Content Analysis in Mammalian Cells
  • Development of Comparative, Cell-based, Reversible p53:Mdm2 and p53:Mdm4 High-Content Fluorescent Two-Hybrid (F2H) Assays for Drug Screening
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Topics: Chromobodies, F2H assay, protein-protein interaction, p53/Mdm2, p53/Mdm4, PPI, PPI inhibitor, cellular PPI assay, PPI dynamics

Abs are Fab!

Posted by Joerg Katzenberger on Jun 5, 2013 4:06:00 PM

This week's Nature product focus features antibodies - if you are reading this, you are of course well aware of the importance of antibodies in today's research. 

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Topics: Chromobodies

This year's winner of the "Industriepreis": Chromotek!

Posted by Joerg Katzenberger on May 15, 2013 5:29:00 PM

Each year, the "Huber Verlag" for new media gathers independent scientists and thought leaders to vote for the most innovative new products in the areas of:

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Topics: Chromobodies

ChromoTek's Chromobody® Technology for HC drug profiling licensed to Bayer

Posted by Katrin Schmidthals on Mar 18, 2013 11:21:00 AM

ChromoTek GmbH (Munich, Germany) has announced today the signing of a license agreement granting Bayer Pharma AG (Bayer) the right to use ChromoTek's proprietary Cell Cycle Chromobody® Technology for drug profiling by High Content Analysis. Under the terms of the agreement, Bayer will apply Chromobody® technology for real time monitoring of cell cycle progression in drug discovery. Financial terms of the agreement were not disclosed. This agreement follows a collaboration in which ChromoTek provided custom cell line development.

ChromoTek's Chromobody® Technology is a patented, universal live cell imaging technology for high content analysis. Chromobodies are single domain antibodies that have been genetically fused to fluorescent proteins to serve as functional nanoprobes in living cells. The Chromobody® technology allows for the first time to trace endogenous intracellular antigens and to visualize dynamic changes of these targets within living cells. Currently available Chromobodies trace important cellular processes like cell cycle, apoptosis, DNA damage and repair or allow cytoskeletal analysis. The Cell Cycle Chromobody® cell line licensed by Bayer traces the complete cell cycle in real time in live cells in a non-invasive manner while providing full flexibility to multiplex with other procedures.

Dr. Kourosh Zolghadr, Head of R&D at ChromoTek comments: "We are pleased to offer our customers efficient tools for achieving more detailed and physiologically relevant information on the effects of drug candidates on cell cycle progression."

About ChromoTek

ChromoTek products set new benchmarks for cellular research. Established in 2008 as a spin-off from Munich’s Ludwig Maximilian University and located in Martinsried, Germany’s leading biotech cluster, ChromoTek develops and markets Chromobody® based live cell assays for drug screening and target validation. Other products include immunological and bioimaging reagents such as the GFP- Trap® for the rapid pull-down of GFP fusion proteins, GFP and RFP-Booster to amplify fluorescence signals of GFP or RFP fusion proteins. ChromoTek’s newly developed fluorescence based protein protein interaction assay called Fluorescent-2-Hybrid (F2H® assay) is available as a development service. More than 2.800 customers from all over the world trust in ChromoTek products.

Download Press release

Posted by Katrin on Mar 18, 2013

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Topics: Chromobodies, Nano-Traps, Nano-Booster, F2H assay, GFP-Trap, Nano-Trap, high content analysis, HCA, Chromobody cell line, Cell Cycle Chromobody

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