GFP (green fluorescent protein): Properties, origin, specifications, tips
Topics: GFP, GFP VHH, GFP Nanobody, EGFP
GFP and RFP-Booster for better immunofluorescence imaging
GFP-Booster and RFP-Booster conjugated to Alexa Fluor® 488, 568 and 647:
- Stronger signal in confocal and standard fluorescence microscopy
- More options in super-resolution microscopy including STORM and MINFLUX
- Constant degree of labeling (DOL = 2 dyes per Booster) for higher resolution
- More than 95% labeling efficiency
Topics: GFP-Booster, RFP-Booster, GFP Nanobody, RFP Nanobody, EGFP
Introduction
Fluorescent proteins (FPs) have been used as protein tags since the mid-1990s mainly for cell biology and fluorescence microscopy. These tags have not only revolutionized cell biology by enabling the imaging of almost any protein, they are also used in biochemical applications. An important example is the immunoprecipitation and affinity purification of FP-tagged proteins, which was enabled by the development of affinity resins with high yield, purity, and affinity such as ChromoTek’s Nano-Traps (https://www.chromotek.com/products/detail/product-detail/nano-traps/).
In this blog we provide a review of
Topics: Immunoprecipitation, mCherry, Immunofluorescence, VHH, Nanobody, mNeonGreen, GFP, GFP Nanobody, GFP Antibody, TurboGFP, EGFP, Western blot, SNAP
Working with green fluorescent proteins: Tools and properties
Jellyfish Green Fluorescent Protein (GFP) and its derivatives are still the most frequently used fluorescent proteins in biomedical research. Recently, additional green fluorescent proteins have been discovered in higher animals such as crustaceans and lancelets. These FPs share a common fold, but diverge widely in their primary sequence. Thus, they require novel, dedicated antibody research tools. Here is an overview about EGFP (the most commonly used GFP derivative), TurboGFP and mNeonGreen.
Topics: Nanobody, mNeonGreen, mNeonGreen immunoprecipitation, GFP, GFP Immunoprecipitation, TurboGFP, TurboGFP immunoprecipitation, EGFP