GFP (green fluorescent protein): Properties, origin, specifications, tips
Topics: GFP, GFP VHH, GFP Nanobody, EGFP
A new Swiss army knife for immunofluorescence: Chimeric heavy chain antibodies
Nanobody-Fc fusion - An alternative Nanobody format that combines its special epitope binding properties with the detection options for Fc-domains
What are heavy chain antibodies?
Topics: Immunofluorescence, VHH, Nanobody, mNeonGreen, GFP, TurboGFP, Antibody, Vimentin
Seizing the day: How to save time on your immunoassay experiments
When time in the lab is limited, learn how to save precious hours on your immunoprecipitation, immunofluorescence and western blotting experiments.
Due to the current situation with the COVID-19 pandemic, many researchers around the world are faced with restrictions on time and resources in the lab. As we adapt to these changes, the pressure is on to find ways to save time without compromising on results. Proteintech and ChromoTek offer solutions to save you hours on your current immunoassay protocols, helping you achieve publication worthy results in a fast and reliable manner.
Topics: Immunoprecipitation, Immunofluorescence, GFP, Nano-Secondaries
Green fluorescent protein (GFP) in plant research
Although the jellyfish Aequorea Victoria Green fluorescent protein (GFP) was already discovered in the 1960s, it took three more decades until it was eventually cloned and could be utilized as a marker protein in E.coli and C. elegans. Since then it has developed into one of the most widely studied and exploited proteins in life sciences. Correspondingly, the importance of GFP was recognized in 2008 when the Nobel Committee awarded Osamu Shimomura, Marty Chalfie, and Roger Tsien the Chemistry Nobel Prize "for the discovery and development of the green fluorescent protein, GFP." |
Topics: GFP, A. Thaliana, plant research
An extract of the published literature since 2016
This blog provides references from the last 4 years in virus research using GFP. Most publications report how immunoprecipitation (IP)/Co-IP of GFP-fusions was conducted to identify host cell binding partners of virus proteins. In addition, mass spectrometry analysis and functional assays have been performed.
Topics: GFP
Mass spec-compatible immunoprecipiation for GFP, mNeonGreen, Myc, RFP, Spot, and TurboGFP
New iST Nano-Trap kits for immunoprecipitation (IP) and sample preparation for mass spectrometry (MS) in just 4 easy steps:
Topics: Immunoprecipitation, Mass spec, Myc-tag, VHH, Nanobody, mNeonGreen, GFP, Spot-Tag, TurboGFP, RFP
iCLIP for the thorough analysis of mRNA:protein interactions
Crystal structure of the anti-GFP VHH-Green Fluorescent Protein complex.
The GFP Nanobody is displayed blue and the GFP in green color.
UV crosslinking techniques are the method of choice for a comprehensive analysis of in-vivo-mRNA targets of an RNA-binding protein (RBP). In the recent publication of Olgeiser et al. (2019), the authors applied individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) to study fungal mRNA transport. For this approach, they have used strains expressing GFP-tagged versions of the two RBPs Grp1 and Rrm4; an optimized protocol was developed to uncover that Grp1 and Rrm4 conjointly bind thousands of shared target messenger ribonucleoproteins (mRNPs) in the fungus U. maydis. The protein:RNA complexes were immunoprecipitated in a multiple detergent containing buffer using ChromoTek’s GFP-Trap Magnetic Agarose. This is a transcriptome‐wide view to an endosomal mRNA transport machinery.
Introduction
Fluorescent proteins (FPs) have been used as protein tags since the mid-1990s mainly for cell biology and fluorescence microscopy. These tags have not only revolutionized cell biology by enabling the imaging of almost any protein, they are also used in biochemical applications. An important example is the immunoprecipitation and affinity purification of FP-tagged proteins, which was enabled by the development of affinity resins with high yield, purity, and affinity such as ChromoTek’s Nano-Traps (https://www.chromotek.com/products/detail/product-detail/nano-traps/).
In this blog we provide a review of
Topics: Immunoprecipitation, mCherry, Immunofluorescence, VHH, Nanobody, mNeonGreen, GFP, GFP Nanobody, GFP Antibody, TurboGFP, EGFP, Western blot, SNAP
Working with green fluorescent proteins: Tools and properties
Jellyfish Green Fluorescent Protein (GFP) and its derivatives are still the most frequently used fluorescent proteins in biomedical research. Recently, additional green fluorescent proteins have been discovered in higher animals such as crustaceans and lancelets. These FPs share a common fold, but diverge widely in their primary sequence. Thus, they require novel, dedicated antibody research tools. Here is an overview about EGFP (the most commonly used GFP derivative), TurboGFP and mNeonGreen.
Topics: Nanobody, mNeonGreen, mNeonGreen immunoprecipitation, GFP, GFP Immunoprecipitation, TurboGFP, TurboGFP immunoprecipitation, EGFP
The best anti-GFP antibody for immunoprecipitation: GFP-Trap
Life science laboratories apply green fluorescent proteins (GFP) to study protein localization, interaction and dynamics in fluorescence microscopy. Immunoprecipitation (IP), mass spectrometry (MS), co-immunoprecipitation (Co-IP) and/or affinity purification investigate more aspects including posttranslational modifications (PTMs), DNA binding, and protein-protein interaction. Here, we compare two different antibody systems for immunoprecipitation of GFP-fusion proteins: GFP-Trap and anti-GFP IgG antibody
Topics: GFP-Trap, GFP, GFP Immunoprecipitation, Best GFP antibody for IP, Best antibody for immunoprecipitation, GFP VHH, GFP Nanobody