ChromoBlog

Fluorescent protein tags

Posted by Christian Linke-Winnebeck on May 28, 2019 5:02:54 PM

Introduction

Fluorescent proteins (FPs) have been used as protein tags since the mid-1990s mainly for cell biology and fluorescence microscopy. These tags have not only revolutionized cell biology by enabling the imaging of almost any protein, they are also used in biochemical applications. An important example is the immunoprecipitation and affinity purification of FP-tagged proteins, which was enabled by the development of affinity resins with high yield, purity, and affinity such as ChromoTek’s Nano-Traps (https://www.chromotek.com/products/detail/product-detail/nano-traps/).

In this blog we provide a review of

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Topics: Immunoprecipitation, mCherry, Immunofluorescence, VHH, Nanobody, mNeonGreen, GFP, GFP Nanobody, GFP Antibody, TurboGFP, EGFP, Western blot, SNAP

Alpaca Nano-Traps for Immunoprecipitation (IP) & Rodent Monoclonals for Western Blot (WB)

Posted by Christoph Eckert on Mar 20, 2017 3:28:14 PM

You obtain the most effective and cleanest IPs of your protein when using ChromoTek’s Nano-Traps (derived from alpaca single domain antibodies). You will see the best protein detetions in WB when applying our highly sensitive monoclonal antibodies.


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Topics: RFP Antibody, GFP Antibody, GST Antibody

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