Although traditional IgG antibodies are often used for immunoprecipitation and protein interaction analysis, GFP-binding protein (GFP VHH, ChromoTek gt-250) and other ChromoTek VHHs easily outcompete those under challenging conditions such as elevated temperatures, varying pH or high denaturant concentrations. In fact, GFP VHH tightly binds GFP-fusion proteins even in 8 M urea at 51 °C, which sets a new limit in protein complex stability, virtually unrivalled by any other capture molecule protein tag pair.
New limit in protein complex stability: GFP-binding protein:GFP complex
Posted by
Klaus Herick on Feb 3, 2017 3:56:34 PM
Topics: GFP-Trap, Nano-Trap, GFP, GFP- Binding Protein