ChromoBlog

GFP-Booster: GFP Nanobody for better images in immunofluorescence

Posted by Christoph Eckert on Jan 31, 2022 1:06:06 PM

Since the discovery of the green fluorescent protein (GFP) it has been widely applied in fluorescence microscopy as a tool to study proteins in their native cellular environment. However, GFP has several intrinsic limitations, such as low signal intensity, fast photo bleaching and signal loss after chemical treatment. The GFP-Booster, which is composed of an anti-GFP Nanobody conjugated to a fluorescent dye, enhances, stabilizes, and re-establishes the signal of GFP fusion proteins in immunofluorescence. In this blog, we answer why the small size of the GFP-Booster is of advantage compared to regular antibodies in immunofluorescence and provide an overview of the GFP variants bound by the GFP-Booster as well as the available dyes.

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Topics: GFP-Booster

GFP and RFP-Booster for better immunofluorescence imaging

Posted by Christoph Eckert on Jan 29, 2020 5:37:07 PM

GFP-Booster and RFP-Booster conjugated to Alexa Fluor® 488, 568 and 647:

  • Stronger signal in confocal and standard fluorescence microscopy
  • More options in super-resolution microscopy including STORM and MINFLUX
  • Constant degree of labeling (DOL = 2 dyes per Booster) for higher resolution
  • More than 95% labeling efficiency
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Topics: GFP-Booster, RFP-Booster, GFP Nanobody, RFP Nanobody, EGFP

ChromoTek Nano-Booster for super resolution microscopy is featured at Biocompare

Posted by Klaus Herick on Jun 2, 2017 4:21:49 PM

ChromoTek’s Marketing Manager Dr. Christoph Eckert has been interviewed by Biocompare, the buyers’ guide for life scientists, on labels for super resolution microscopy.

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Topics: GFP-Booster, RFP-Booster, Super resolution microscopy, VHH, Nanobody

Nano-Booster FAQs

Posted by Larisa Yurlova on Feb 19, 2016 2:48:05 PM

ChromoTek Nano-Boosters are ideal for Super-Resolution and “traditional” fluorescence microscopy because of their high affinity and extremely small size of just 2 to 3 nm. Technically speaking, the GFP-and RFP-Boosters are composed of the highly specific GFP- or RFP-binding domains of alpaca antibodies (also called “nanobodies”), covalently coupled to a selection of fluorescent dyes.

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Topics: Nano-Booster, GFP-Booster, RFP-Booster, GFP Nanobody, RFP Nanobody, mCherry Nanobody

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