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GFP- Nanobodies and RFP- Nanobodies conjugated to quantum dots

Posted by Christoph Eckert on Jul 12, 2018 8:39:33 AM

Chromotek’s GFP- binding protein, an anti- GFP Nanobody, is a very small and effective tool for binding and visualizing GFP-tagged proteins. In their recent publication, Modi et al. successfully functionalized quantum dots with the GFP- Nanobody (QD GFP- Nanobody). Thus, they created a small GFP- specific label with a very strong fluorescent signal.

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Topics: GFP Nanobody, RFP Nanobody, Quantum dots, single particle tracking, Quantum dot Nanobody

On-bead digestion protocol: From immunoprecipitation with nanobodies to MS analysis

Posted by Christoph Eckert on Sep 15, 2017 3:52:48 PM

What makes on-bead digestion favorable?

Just pull down your protein of interest with immobilized nanobodies, also termed VHHs or single domain antibodies. Then follow the on-bead digestion protocol (see below) and submit the digest to your core facility for effective mass spectrometer analysis of (co-) precipitated proteins.

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Topics: GFP Immunoprecipitation, GFP Nanobody, GFP VHH, GFP mass spec, on-bead digestion, GFP-Trap

The best anti-GFP antibody for immunoprecipitation: GFP-Trap

Posted by Christoph Eckert on Sep 7, 2017 1:05:34 PM

Life science laboratories apply green fluorescent proteins (GFP) to study protein localization, interaction and dynamics in fluorescence microscopy. Immunoprecipitation (IP), mass spectrometry (MS), co-immunoprecipitation (Co-IP) and/or affinity purification investigate more aspects including posttranslational modifications (PTMs), DNA binding, and protein-protein interaction. Here, we compare two different antibody systems for immunoprecipitation of GFP-fusion proteins: GFP-Trap and anti-GFP IgG antibody

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Topics: GFP, GFP Immunoprecipitation, GFP-Trap, Best GFP antibody for IP, GFP VHH, GFP Nanobody, Best antibody for immunoprecipitation

Nano-Booster FAQs

Posted by Larisa Yurlova on Feb 19, 2016 2:48:05 PM

ChromoTek Nano-Boosters are ideal for Super-Resolution and “traditional” fluorescence microscopy because of their high affinity and extremely small size of just 2 to 3 nm. Technically speaking, the GFP-and RFP-Boosters are composed of the highly specific GFP- or RFP-binding domains of alpaca antibodies (also called “nanobodies”), covalently coupled to a selection of fluorescent dyes.

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Topics: Nano-Booster, GFP-Booster, RFP-Booster, GFP Nanobody, RFP Nanobody, mCherry Nanobody

ChromoTek's Nanobodies are versatile and proven affinity tools for multiplexed analysis of protein-protein interactions using Luminex® or Biacore™ assay technologies

Posted by Laurentia Tanase on Aug 14, 2015 11:56:12 AM

Benefit from high binding affinities and extraordinary functional stabilities of Nanobodies

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Topics: GFP Nanobody, GFP-Trap, GST Nanobody

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