When time in the lab is limited, learn how to save precious hours on your immunoprecipitation, immunofluorescence and western blotting experiments.
Due to the current situation with the COVID-19 pandemic, many researchers around the world are faced with restrictions on time and resources in the lab. As we adapt to these changes, the pressure is on to find ways to save time without compromising on results. Proteintech and ChromoTek offer solutions to save you hours on your current immunoassay protocols, helping you achieve publication worthy results in a fast and reliable manner.
Read More
Topics:
Immunoprecipitation,
Immunofluorescence,
GFP,
Nano-Secondaries
ChromoTek's Nano-Traps are optimized for the immunoprecipitation (IP) of proteins and their interacting factors. Nano-Traps comprise of a Nanobody/ VHH conjugated to beads. For 3 Nano-Traps, e.g. GFP-Trap®, RFP-Trap®, and Spot-Trap®, we offer different types of beads:
- Agarose beads
- Magnetic Agarose beads
- Dynabeads™
Immunoprecipitation with GFP-Trap.
I: Input, FT: Flow-Through, B: Bound
Based on the properties of the different beads (see table), we recommend:
- Agarose beads for very low background and high binding capacity IP
- Magnetic Agarose beads for magnetic separation and high binding capacity IP
- Dynabeads for IP of very large proteins/complexes
Read More
Topics:
GFP-Trap,
Immunoprecipitation,
RFP-Trap,
GFP Immunoprecipitation,
GFP Nanobody,
Spot-Trap,
Dynabeads
Introduction of Affinity vs. Specificity
Read More
Topics:
Immunoprecipitation
Introduction
A high background from unspecific binding of proteins is a common problem in immunoprecipitation (IP). In this blog, the origin of background and different optimization strategies are discussed. Furthermore, it is shown how you can achieve a low background when using the ChromoTek GFP-Trap®, a ready to use reagent for IP of a GFP-tagged proteins.
Read More
Topics:
GFP-Trap,
Immunoprecipitation
Estimated reading time: 3 minutes
Read More
Topics:
Immunoprecipitation
The pull-down of proteins is an established technology. It can be difficult, particularly when the protein of interest is expressed at low levels. Here we discuss 5 tips that help you to improve your IP results.
Read More
Topics:
Immunoprecipitation,
GFP Immunoprecipitation
Affinity tags are valuable tools for immunoprecipitation (IP). Fused to the protein of interest, they enable proteome-wide identification of interaction partners by mass spectrometry.
Read More
Topics:
Immunoprecipitation,
Mass spec
New iST Nano-Trap kits for immunoprecipitation (IP) and sample preparation for mass spectrometry (MS) in just 4 easy steps:
Read More
Topics:
Immunoprecipitation,
Mass spec,
Myc-tag,
VHH,
Nanobody,
mNeonGreen,
GFP,
Spot-Tag,
TurboGFP,
RFP
Introduction
Fluorescent proteins (FPs) have been used as protein tags since the mid-1990s mainly for cell biology and fluorescence microscopy. These tags have not only revolutionized cell biology by enabling the imaging of almost any protein, they are also used in biochemical applications. An important example is the immunoprecipitation and affinity purification of FP-tagged proteins, which was enabled by the development of affinity resins with high yield, purity, and affinity such as ChromoTek’s Nano-Traps (https://www.chromotek.com/products/detail/product-detail/nano-traps/).
In this blog we provide a review of
Read More
Topics:
Immunoprecipitation,
mCherry,
Immunofluorescence,
VHH,
Nanobody,
mNeonGreen,
GFP,
GFP Nanobody,
GFP Antibody,
TurboGFP,
EGFP,
Western blot,
SNAP
A collaboration between ChromoTek GmbH and Ludwig-Maximilians-University Munich (Germany) has yielded a novel nanobody that sheds new light on an important protein complex called INO80. This work was recently published in the high-impact journal Nature Structural & Molecular Biology (Knoll et al. 2018).
Read More
Topics:
Immunoprecipitation,
Nanobody,
Crystallisation