ChromoBlog

What is antibody specificity and affinity? And why is it important for immunoprecipitation?

Posted by Dr. Astrid Sitte on Mar 12, 2020 5:17:58 PM

Introduction of Affinity vs. Specificity

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Topics: Immunoprecipitation

How to obtain a low background in immunoprecipitation assays

Posted by Dr. Astrid Sitte on Mar 10, 2020 2:02:57 PM

Introduction

A high background from unspecific binding of proteins is a common problem in immunoprecipitation (IP). In this blog, the origin of background and different optimization strategies are discussed. Furthermore, it is shown how you can achieve a low background when using the ChromoTek GFP-Trap®, a ready to use reagent for IP of a GFP-tagged proteins.

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Topics: GFP-Trap, Immunoprecipitation

Immunoprecipitation without additional bands

Posted by Dr. Astrid Sitte on Mar 6, 2020 3:03:09 PM

Estimated reading time:  3 minutes

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Topics: Immunoprecipitation

5 Tips for Successful Immunoprecipitations (IP)

Posted by Klaus Herick on Mar 3, 2020 3:15:19 PM

The pull-down of proteins is an established technology. It can be difficult, particularly when the protein of interest is expressed at low levels. Here we discuss 5 tips that help you to improve your IP results.

 

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Topics: Immunoprecipitation, GFP Immunoprecipitation

Multiple kits for streamlined affinity purification mass spectrometry (AP-MS)

Posted by Dr. Fabian Hosp (PreOmics) on Feb 24, 2020 10:13:00 AM

Affinity tags are valuable tools for immunoprecipitation (IP). Fused to the protein of interest, they enable proteome-wide identification of interaction partners by mass spectrometry.

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Topics: Immunoprecipitation, Mass spec

Mass spec-compatible immunoprecipiation for GFP, mNeonGreen, Myc, RFP, Spot, and TurboGFP

Posted by Christoph Eckert on Feb 20, 2020 1:12:28 PM

New iST Nano-Trap kits for immunoprecipitation (IP) and sample preparation for mass spectrometry (MS) in just 4 easy steps:

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Topics: Immunoprecipitation, Mass spec, Myc-tag, VHH, Nanobody, mNeonGreen, GFP, Spot-Tag, TurboGFP, RFP

Fluorescent protein tags

Posted by Christian Linke-Winnebeck on May 28, 2019 5:02:54 PM

Introduction

Fluorescent proteins (FPs) have been used as protein tags since the mid-1990s mainly for cell biology and fluorescence microscopy. These tags have not only revolutionized cell biology by enabling the imaging of almost any protein, they are also used in biochemical applications. An important example is the immunoprecipitation and affinity purification of FP-tagged proteins, which was enabled by the development of affinity resins with high yield, purity, and affinity such as ChromoTek’s Nano-Traps (https://www.chromotek.com/products/detail/product-detail/nano-traps/).

In this blog we provide a review of

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Topics: Immunoprecipitation, mCherry, Immunofluorescence, VHH, Nanobody, mNeonGreen, GFP, GFP Nanobody, GFP Antibody, TurboGFP, EGFP, Western blot, SNAP

ChromoTek’s Nanobody helps LMU scientists in their quest to understand a giant protein complex

Posted by Christian Linke-Winnebeck on Sep 20, 2018 3:42:47 PM

A collaboration between ChromoTek GmbH and Ludwig-Maximilians-University Munich (Germany) has yielded a novel nanobody that sheds new light on an important protein complex called INO80. This work was recently published in the high-impact journal Nature Structural & Molecular Biology (Knoll et al. 2018).

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Topics: Immunoprecipitation, Nanobody, Crystallisation

TurboGFP: Properties, Sequence, MW, origin

Posted by Christoph Eckert on Jul 3, 2018 1:23:20 PM

TurboGFP is a bright green fluorescent protein used to study protein function, localization and dynamics in cells. Nanobody based research tools allow reproducible biochemical analysis including mass spectrometry and enzyme activity measurements of TurboGFP fusion proteins.

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Topics: Immunoprecipitation, TurboGFP, TurboGFP immunoprecipitation, Best TurboGFP antibody, TurboGFP Nanobody

Affinity purification, depletion, and immunoprecipitation of GST-tagged proteins

Posted by Klaus Herick on Jul 6, 2017 1:30:48 PM

Multiple literature references for GST-Trap


This article provides an overview of scientific publications that reference the ChromoTek GST-Trap superior binding performance for various applications. These include immunoprecipitation (IP), Co-IP of Glutathione S-Transferase (GST)-fusion proteins, and Luminex bead assays of GST-tagged and GFP-tagged proteins. Besides, usages for depletion of GST (glutathione sepharose)  after cleavage from recombinant fusion proteins and affinity purification of GST-tagged proteins are possible.

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Topics: Immunoprecipitation, GST, affinity resin, GST immunoprecipitation, references, affinity purification, GST pulldown

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