Nanobody-Fc fusion - An alternative Nanobody format that combines its special epitope binding properties with the detection options for Fc-domains
New iST Nano-Trap kits for immunoprecipitation (IP) and sample preparation for mass spectrometry (MS) in just 4 easy steps:
Topics: Immunoprecipitation, Mass spec, Myc-tag, VHH, Nanobody, mNeonGreen, GFP, Spot-Tag, TurboGFP, RFP
Introduction
Fluorescent proteins (FPs) have been used as protein tags since the mid-1990s mainly for cell biology and fluorescence microscopy. These tags have not only revolutionized cell biology by enabling the imaging of almost any protein, they are also used in biochemical applications. An important example is the immunoprecipitation and affinity purification of FP-tagged proteins, which was enabled by the development of affinity resins with high yield, purity, and affinity such as ChromoTek’s Nano-Traps (https://www.chromotek.com/products/detail/product-detail/nano-traps/).
In this blog we provide a review of
Topics: Immunoprecipitation, mCherry, Immunofluorescence, VHH, Nanobody, mNeonGreen, GFP, GFP Nanobody, GFP Antibody, TurboGFP, EGFP, Western blot, SNAP
Jellyfish Green Fluorescent Protein (GFP) and its derivatives are still the most frequently used fluorescent proteins in biomedical research. Recently, additional green fluorescent proteins have been discovered in higher animals such as crustaceans and lancelets. These FPs share a common fold, but diverge widely in their primary sequence. Thus, they require novel, dedicated antibody research tools. Here is an overview about EGFP (the most commonly used GFP derivative), TurboGFP and mNeonGreen.
Topics: Nanobody, mNeonGreen, mNeonGreen immunoprecipitation, GFP, GFP Immunoprecipitation, TurboGFP, TurboGFP immunoprecipitation, EGFP
Recently a new bright monomeric yellow-green fluorescent protein has been published, which is called mNeonGreen. This protein has already been frequently used for mainly microscopic applications in both wide-field microscopy and super resolution microscopy. What is mNeonGreen all about?
Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen Western blot, mNeonGreen VHH, mNeonGreen immunoprecipitation, mNeonGreen immunofluorescence, GFP, mNeonGreen sequence
ChromoTek is proud to introduce new tools for mNeonGreen fusion proteins:
- anti-mNeonGreen mouse monoclonal antibody 32F6 for immunofluorescence (IF) and Western blot (WB)
- mNeonGreen-Trap for immunoprecipitation (IP) of mNeonGreen fusion proteins. The mNeonGreen-Trap is based on an anti-mNeonGreen VHH (mNeonGreen binding protein)
Click here and request a free sample:
Topics: mNeonGreen, mNeonGreen Antibody, mNeonGreen Western blot, mNeonGreen VHH, mNeonGreen-Trap, mNeonGreen immunoprecipitation, mNeonGreen immunofluorescence
