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Characterization of COVID-19 antibodies by Bio-Layer Interferometry using Nano-CaptureLigands

Posted by Dr. Astrid Sitte on Jul 8, 2020 9:10:21 AM

Characterization of antibodies

Essential aspects of how antibodies (Abs) bind to their targets are their affinity and binding kinetics. In order to determine these parameters, different biophysical methods such as Surface Plasmon Resonance (SPR), Bio-Layer Interferometry (BLI), MicroScale Thermophoresis (MST), or Enzyme-linked Immunosorbent Assay (ELISA) can be applied. Some of these methods require the immobilization of the antibody on a surface and the titration of the target antigen to measure the dissociation constant KD and the association and dissociation rates ka and kd (also called on- and off rates kon and koff).

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Topics: Nano-CaptureLigands

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