ChromoBlog

Seizing the day: How to save time on your immunoassay experiments

Posted by Dr. Rebecca Northeast (Proteintech) on Feb 3, 2021 10:59:58 AM

When time in the lab is limited, learn how to save precious hours on your immunoprecipitation, immunofluorescence and western blotting experiments.

Due to the current situation with the COVID-19 pandemic, many researchers around the world are faced with restrictions on time and resources in the lab. As we adapt to these changes, the pressure is on to find ways to save time without compromising on results. Proteintech and ChromoTek offer solutions to save you hours on your current immunoassay protocols, helping you achieve publication worthy results in a fast and reliable manner.

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Topics: Immunoprecipitation, Immunofluorescence, GFP, Nano-Secondaries

The importance of blocking when using Nano-Secondaries for IF

Posted by Dr. Astrid Sitte on Jan 28, 2020 5:04:30 PM

Introduction

Blocking is an essential step during the preparation of a sample for immunofluorescence detection. Blocking improves the sensitivity by reducing nonspecific background and therefore increases image quality. Insufficient blocking results in higher background noise and over-blocking can even mask the specific signal.

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Topics: Nano-Secondaries, staining

What is one-step immunostaining?

Posted by Dr. Astrid Sitte on Jul 26, 2019 2:45:28 PM

Simultaneous immunostaining, also called one-step immunostaining vs. sequential immunostaining. Nano-Secondaries stain different primary antibodies equally well in one-step staining and sequential staining.

HeLa cells were immunostained with different primary antibodies and Nano-Secondaries Alexa Fluor® 647 (1:1,000, magenta). Cell nuclei were stained with DAPI (blue). Scale bar, 20 μm.

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Topics: live cell imaging, Secondary antibody, Nano-Secondaries, One-step immunostaining, staining

Important facts to know when using Nano-Secondaries

Posted by Klaus Herick on Jul 23, 2019 1:27:20 PM

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Topics: VHH, Nanobody, Secondary antibody, Nano-Secondaries, Alexa Fluor, One-step immunostaining

Advantages of recombinant Nano-Secondaries

Posted by Klaus Herick on May 23, 2019 4:10:12 PM

Nano-Secondaries are monoclonal Nanobodies that bind to primary antibodies in a species and subtype specific manner, i.e. Nano-Secondaries are very precise secondary antibodies. They are recombinantly produced in bacterial or other animal-free expression systems; in contrast, classical mono- or polyclonal secondary antibodies are traditionally produced using hybridoma technologies or are isolated from a host’s blood.

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Topics: Nanobody, Secondary antibody, Validation, recombinat expression, Nano-Secondaries

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