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Analyze protein interactions live in mammalian cells

Posted by Christoph Eckert on May 16, 2017 4:18:31 PM

  • Do you have GFP or RFP protein constructs and/or libraries?
  • Do you want to screen agonists or antagonists?
  • Do you want to save time and start your experiment without method set-up?

Just do it. All you need is the ChromoTek F2H Kit Basic: Transfect the bait and the prey plasmids with the Platform Reagent into the F2H cells. Image and quantify your results already at the next day.

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Topics: F2H assay, PPI

ChromoTek Introduces the new F2H® Kit for Protein-Protein Interaction Analysis

Posted by Larisa Yurlova on Mar 31, 2014 5:33:00 PM

ChromoTek GmbH (Martinsried, Germany) announces the launch of a new research assay for protein-protein interaction (PPI)analysis in live mammalian cells, the Fluorescence Two-Hybrid (F2H®) Kit. This new product is the result of Chromotek's long experience with applying the F2H technology in PPI compound screening for pharmaceutical and biotech customers. The new kit format overcomes limitations of currently available methods and will fascinate biomedical scientists, who investigate protein-protein interactions (PPIs) by its ease of use.

ChromoTek’s F2H® Kit enables effortless analysis of interactions between any GFP- and RFP- tagged protein pairs in living mammalian cells by conventional fluorescence microscopy. It is a fast, simple and quantitative way to characterize PPIs in intracellular environment, screen for PPI inhibitors and evaluate their activity in real time. Unlike available complementation assays, such as split-YFP, F2H® is a fully reversible assay and therefore better suitable for testing PPI antagonists. In comparison to FRET assays, the ingeniously simple optical read-out of F2H® does not require sophisticated equipment, making it much more affordable and at the same time robust and reliable.

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Topics: F2H assay, HCA, protein-protein interaction, p53/Mdm2, p53/Mdm4, PPI, PPI inhibitor, cellular PPI assay, PPI dynamics

Protein-protein interactions: see them, screen then, publish!

Posted by Larisa Yurlova on Mar 20, 2014 1:40:00 PM

Yes! Our latest paper reporting on screening for protein-protein interaction inhibitors with F2H is now online: http://www.ncbi.nlm.nih.gov/pubmed/24476585.

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Topics: F2H assay, p53/Mdm2, p53/Mdm4, protein-protein interaction, PPI, PPI inhibitor, cellular PPI assay, PPI dynamics

We present two posters at #MipTec2013 in Basel

Posted by Joerg Katzenberger on Sep 23, 2013 9:56:00 PM

 
  • Intracellular Fluorescent Alpaca Antibodies (Chromobodies):
    Non-invasive Biomarkers for High-Content Analysis in Mammalian Cells
  • Development of Comparative, Cell-based, Reversible p53:Mdm2 and p53:Mdm4 High-Content Fluorescent Two-Hybrid (F2H) Assays for Drug Screening
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Topics: Chromobodies, F2H assay, p53/Mdm2, p53/Mdm4, protein-protein interaction, PPI, PPI inhibitor, cellular PPI assay, PPI dynamics

ChromoTek showing a poster about its latest developments:

Posted by Katrin Schmidthals on Jan 4, 2012 2:10:00 AM


High Content Analysis Meeting 2012  in  San Francisco. January 10-13, 2012 at 
The Fairmont Hotel
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Topics: Chromobodies, USA, Nano-Booster, F2H assay, protein-protein interaction, PPI, GFP-Trap, Nano-Trap

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