Using both Proteintech and Chromotek antibodies, Davy and colleagues published a landmark paper in Science late in 2020, detailing how Neuropilin-1 is required for SARS-CoV-2 host cell entry.
ChromoTek's Nano-Traps are optimized for the immunoprecipitation (IP) of proteins and their interacting factors. Nano-Traps comprise of a Nanobody/ VHH conjugated to beads. For 3 Nano-Traps, e.g. GFP-Trap®, RFP-Trap®, and Spot-Trap®, we offer different types of beads:
- Agarose beads
- Magnetic Agarose beads
- Magnetic Particles M-270
Immunoprecipitation with GFP-Trap.
I: Input, FT: Flow-Through, B: Bound
Based on the properties of the different beads (see table), we recommend:
- Agarose beads for very low background and high binding capacity IP
- Magnetic Agarose beads for magnetic separation and high binding capacity IP
- Magnetic Particles M-270 for IP of very large proteins/complexes
Topics: GFP-Trap, Immunoprecipitation, RFP-Trap, GFP Immunoprecipitation, GFP Nanobody, Spot-Trap
See the advantages of ChromoTek’s RFP-Trap in ChIP applications as demonstrated by Dr. Caroline Rivers, Laboratory for Integrative Neuroscience & Endocrinology, University of Bristol. In absence of suitable antibodies for an inducible hormone receptor, mCherry has been fused to the N-terminus of the receptor. In order to effectively pull down the mCherry fusion protein DNA complex, ChromoTek's RFP-Trap was used.
Topics: Nano-Traps, ChIP, mCherry, RFP-Trap
