ChromoBlog

Mass spec-compatible immunoprecipiation for GFP, mNeonGreen, Myc, RFP, Spot, and TurboGFP

Posted by Christoph Eckert on Feb 20, 2020 1:12:28 PM

New iST Nano-Trap kits for immunoprecipitation (IP) and sample preparation for mass spectrometry (MS) in just 4 easy steps:

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Topics: Immunoprecipitation, Mass spec, Myc-tag, VHH, Nanobody, mNeonGreen, GFP, Spot-Tag, TurboGFP, RFP

How to overcome the limitations of antibody-based affinity resins for protein purification

Posted by Dr. Astrid Sitte on Dec 18, 2019 2:19:42 PM

Introduction

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Topics: affinity resin, affinity purification, Spot-Tag, Spot Nanobody, protein purification, epitope tag

Spot Capture and Detection Peptide Tag and Nanobody

Posted by Klaus Herick on Sep 9, 2019 1:37:29 PM

The Spot-Nanobody (green) binds to the Spot-Tag sequence motif PDRVRAVSHWSS. Upon binding, the Spot-Tag peptide is embedded on the surface of the Spot-Nanobody and becomes a β-sheet extension of the Spot-VHH. Defined interactions of the Spot-Nanobody’s side chains to the Spot-peptide determine specificity. In addition, the Spot-peptide is clamped by two amino acid side chains of the Spot-Nanobody. This binding mechanism elucidates why the Spot-Nanobody binds with high affinity to the Spot-Tag.

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Topics: Spot-Tag, Spot Nanobody, Spot-Label

First Peptide-tag specific Nanobody applied in super resolution microscopy (SRM)

Posted by Klaus Herick on Mar 2, 2018 11:02:00 AM

You may have noticed the recent publication “A peptide tag-specific nanobody enables high-quality labeling for STORM imaging” of Virant et al (2018) in Nature Communications doi: 10.1038/s41467-018-03191-2, where for the first time a peptide-tag specific Nanobody was applied in dSTORM imaging: The authors have described and discussed the performance of a BC2 peptide-tag specific Nanobody to image BC2 peptide tag fusion proteins in super resolution microscopy, i.e. dSTORM. This Blog features that BC2 peptide-tag specific Nanobody, which ChromoTek markets under the names Spot-Label® and Spot-Trap®. In addition, this Blog figures the significantly improved version of the BC2 peptide -tag, which is called Spot-Tag®.

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Topics: Nanobody, Spot-Tag, Capture/detection System, Spot Nanobody, Spot-Trap, Spot-Label, Spot super resolution microscopy, Spot STED, Spot immunofluorescence, BC2tag

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