ChromoTek's Nano-Traps are optimized for the immunoprecipitation (IP) of proteins and their interacting factors. Nano-Traps comprise of a Nanobody/ VHH conjugated to beads. For 3 Nano-Traps, e.g. GFP-Trap®, RFP-Trap®, and Spot-Trap®, we offer different types of beads:
- Agarose beads
- Magnetic Agarose beads
- Magnetic Particles M-270
Immunoprecipitation with GFP-Trap.
I: Input, FT: Flow-Through, B: Bound
Based on the properties of the different beads (see table), we recommend:
- Agarose beads for very low background and high binding capacity IP
- Magnetic Agarose beads for magnetic separation and high binding capacity IP
- Magnetic Particles M-270 for IP of very large proteins/complexes