ChromoBlog

SNAP-tag and CLIP-tag

Posted by Christoph Eckert on May 7, 2020 9:34:17 AM

SNAP-tag and CLIP-tag are self-labeling protein tags. The SNAP-tag protein and the CLIP-tag protein can be fused to a protein of interest (POI) and used for cellular and biochemical analysis.

Read More

Topics: VHH, Nanobody, SNAP

HaloTag

Posted by Christoph Eckert on May 5, 2020 2:47:26 PM

HaloTag is a protein tag. The HaloTag protein can be fused to your protein of interest (POI) which enables it for cellular and biochemical analysis. It can also be used for live cell imaging.

Read More

Topics: VHH, Nanobody

Mass spec-compatible immunoprecipiation for GFP, mNeonGreen, Myc, RFP, Spot, and TurboGFP

Posted by Christoph Eckert on Feb 20, 2020 1:12:28 PM

New iST Nano-Trap kits for immunoprecipitation (IP) and sample preparation for mass spectrometry (MS) in just 4 easy steps:

Read More

Topics: Immunoprecipitation, Mass spec, Myc-tag, VHH, Nanobody, mNeonGreen, GFP, Spot-Tag, TurboGFP, RFP

Bispecific T cell engagers

Posted by Christoph Eckert on Sep 13, 2019 1:04:52 PM
Bispecific T cell engagers are bispecific antibodies designed to link T cells with cancer cells. The simultaneous binding of the bispecific antibody to T cell and tumor cell initiates the activation of the T cell. For this purpose, the bispecific antibody carries two antigen binding domains against the T cell (often CD3 T cell receptor) and against a cell surface protein of the tumor cell.

ChromoTek offers two bispecific T cell engagers:

Read More

Topics: VHH, Nanobody, Bispecific, T cell

Important facts to know when using Nano-Secondaries

Posted by Klaus Herick on Jul 23, 2019 1:27:20 PM

Read More

Topics: VHH, Nanobody, Secondary antibody, Nano-Secondaries, Alexa Fluor, One-step immunostaining

Fluorescent protein tags

Posted by Christian Linke-Winnebeck on May 28, 2019 5:02:54 PM

Introduction

Fluorescent proteins (FPs) have been used as protein tags since the mid-1990s mainly for cell biology and fluorescence microscopy. These tags have not only revolutionized cell biology by enabling the imaging of almost any protein, they are also used in biochemical applications. An important example is the immunoprecipitation and affinity purification of FP-tagged proteins, which was enabled by the development of affinity resins with high yield, purity, and affinity such as ChromoTek’s Nano-Traps (https://www.chromotek.com/products/detail/product-detail/nano-traps/).

In this blog we provide a review of

Read More

Topics: Immunoprecipitation, mCherry, Immunofluorescence, VHH, Nanobody, mNeonGreen, GFP, GFP Nanobody, GFP Antibody, TurboGFP, EGFP, Western blot, SNAP

ChromoTek Nano-Booster for super resolution microscopy is featured at Biocompare

Posted by Klaus Herick on Jun 2, 2017 4:21:49 PM

ChromoTek’s Marketing Manager Dr. Christoph Eckert has been interviewed by Biocompare, the buyers’ guide for life scientists, on labels for super resolution microscopy.

Read More

Topics: GFP-Booster, RFP-Booster, Super resolution microscopy, VHH, Nanobody

News about Nanobodies

For researchers interested in

  • New Nanobody based tools
  • Tips and tricks
  • Featured publications

Recent Posts

Posts by Tag

See all