ChromoBlog

Virus research using GFP

Posted by Dr. Astrid Sitte on Mar 26, 2020 1:05:30 PM

An extract of the published literature since 2016

This blog provides references from the last 4 years in virus research using GFP. Most publications report how immunoprecipitation (IP)/Co-IP of GFP-fusions was conducted to identify host cell binding partners of virus proteins. In addition, mass spectrometry analysis and functional assays have been performed.

virus

In earlier research on corona viruses (not CoVID19, but previous viruses of the corona strain) our GFP-Trap has been applied. The results of the research work have been published in these references:

This table shows an overview of literature references, in which the GFP-Trap has been used for virus research:

Virus Species

Genus

Family

Application

Pubmed ID / Link

Year

Abutilon mosaic virus (AbMV)
Pea necrotic yellow dwarf virus (PNYDV)

Begomovirus
Nanovirus

Geminiviridae
Nanoviridae

Co-IP, MS

27693920

2017

Adenovirus

n/a

Adenoviridae

Co-IP, MS

27307198

2016

Alphacoronavirus

Alphacoronavirus

Coronaviridae

Co-IP

27479465

2016

Cauliflower mosaic virus

Caulimovirus

Caulimoviridae

Co-IP

30840696

2019

Chikungunya virus (CHIKV)

Togaviridae

Alphavirus

Co-IP, MS

29702546

2018

Chikungunya virus (CHIKV)

Togaviridae

Alphavirus

IP, functional assays

https://doi.org/10.1101/2020.01.07.896977

2020

Chikungunya virus (CHIKV)
Semliki Forest virus (SFV)

Togaviridae
Togaviridae

Alphavirus
Alphavirus

Co-IP

31199850

2019

Citrus psorosis virus (CPsV)

Ophiovirus

Aspiviridae

Co-IP

30135122

2018

Cotton leaf curl Multan virus (CLCuMuV)

Begomovirus

Geminiviridae

Co-IP

27315204

2016

Cotton leaf curl Multan virus (CLCuMuV)
Tomato yellow leaf curl virus (TYLCV)
Tomato yellow leaf curl China virus (TYLCCNV)

Begomovirus
Begomovirus
Begomovirus

Geminiviridae
Geminiviridae
Geminiviridae

Co-IP

28244873

2017

Dengue virus (DENV)

Flavivirus

Flaviviridae

Co-IP

27076639

2016

Dengue virus (DENV)
Poliovirus (PV)
Zika virus (ZIKV)

Flavivirus
Enterovirus
Flavivirus

Flaviviridae
Picornaviridae
Flaviviridae

Co-IP, MS

30608919

2019

Ebola virus

Ebolavirus

Filoviridae

Co-IP

29290611

2018

Ebola virus (EBOV)

Ebolavirus

Filoviridae

Co-IP, MS

27786485

2016

Enterovirus

Enterovirus

Picornaviridae

Co-IP

31381608

2019

Guinea pig cytomegalovirus (GPCMV)

Cytomegalovirus

Betaherpesvirinae

Co-IP

28651121

2017

Guinea pig cytomegalovirus (GPCMV)

Cytomegalovirus

Betaherpesvirinae

Co-IP

27387220

2016

Hendra virus V (HeV)

Henipavirus

Paramyxoviridae

Co-IP

29321677

2018

Hepatitis C virus (HCV)

Hepacivirus

Flaviviridae

IP

29352312

2018

Herpes simplex virus (HSV)

Simplexvirus

Herpesviridae

Co-IP

28507315

2017

Herpes simplex virus type 1 (HSV-1)

Simplexvirus

Herpesviridae

Co-IP

27852850

2017

Herpes simplex virus type 1 (HSV-1)

Simplexvirus

Herpesviridae

Co-IP

https://doi.org/10.1101/810663

2019

Herpes simplex virus type 1 (HSV-1)

Simplexvirus

Herpesviridae

Co-IP, MS

https://doi.org/10.1101/729343

2019

Herpes simplex virus type 1 (HSV-1)
Vaccinia virus (VacV)

Simplexvirus
Orthopoxvirus

Herpesviridae
Poxviridae

Co-IP, RIP

28636603

2017

Herpes simplex virus type 2 (HSV-2)

Simplexvirus

Herpesviridae

Co-IP

30080903

2018

Human cytomegalovirus (HCMV)

Cytomegalovirus

Betaherpesvirinae

Co-IP

27181750

2016

Human immunodeficiency virus (HIV)

Lentivirus

Orthoretrovirinae

Co-IP

26792716

2016

Human immunodeficiency virus 1 (HIV-1)

Lentivirus

Orthoretrovirinae

Co-IP

28835492

2017

Human immunodeficiency virus 1 (HIV-1)

Lentivirus

Orthoretrovirinae

Co-IP

27560372

2016

Human immunodeficiency virus 1 (HIV-1)

Lentivirus

Orthoretrovirinae

Co-IP

31776272

2020

Human noroviruses (HuNoV)

Norovirus

Caliciviridae

Co-IP

31403400

2019

Human papillomavirus (HPV)

n/a

Papillomaviridae

IP

29045464

2017

Infectious bronchitis virus (IBV)

Gammacoronavirus

Coronaviridae

Co-IP, MS

23637410

2013

Influenza A virus

Alphainfluenzavirus

Orthomyxoviridae

Co-IP

29061883

2017

Influenza A virus

Alphainfluenzavirus

Orthomyxoviridae

Co-IP

28250123

2017

Kallithea virus (KV)

Alphanudivirus

Nudiviridae

Co-IP, MS

30404807

2019

Kaposi's sarcoma-associated herpesvirus (KSHV)

Rhadinovirus

Herpesviridae

Co-IP

27798559

2016

Kaposi's sarcoma-associated herpesvirus (KSHV)

Rhadinovirus

Herpesviridae

Co-IP, MS

26811480

2016

Merkel cell polyomavirus (MCPyV)

Alphapolyomavirus

Polyomaviridae

Co-IP

28445980

2017

Nipah virus (NiV)

Henipavirus

Paramyxoviridae

Co-IP

27622322

2016

Plum Pox Virus

Potyvirus

Potyviridae

Co-IP

27301551

2017

Porcine reproductive and respiratory syndrome virus (PRRSV)

Porartevirus

Arteriviridae

Co-IP

30819256

2019

Porcine reproductive and respiratory syndrome virus (PRRSV)

Porartevirus

Arteriviridae

IP

27327135

2016

Pseudorabies Virus

Varicellovirus

Herpesviridae

ChIP

28283558

2017

Rabies virus (RABV)

Lyssavirus

Rhabdoviridae

Co-IP, AP-MS

29084252

2017

Rabies virus (RABV)

Lyssavirus

Rhabdoviridae

Co-IP

26939125

2016

Red clover necrotic mosaic virus (RCNMV)

Dianthovirus

Tombusviridae

Co-IP

28154139

2017

Reptilian orthoreovirus

Orthoreovirus

Reoviridae

Co-IP

https://doi.org/10.1101/761502

2019

Severe acute respiratory syndrome coronavirus (SARS-CoV)

Betacoronavirus

Coronaviridae

Co-IP, ubiquitination/deubiquitination assay, kinase assay

27519799

2016

Tick-borne encephalitis virus

Flavivirus

Flaviviridae

Co-IP

31243132

2019

Tobacco vein banding mosaic virus (TVBMV)

Potyvirus

Potyviridae

Co-IP

28230184

2017

Tomato yellow leaf curl virus (TYLCV)

Begomovirus

Geminiviridae

Co-IP

28946693

2017

Tomato yellow leaf curl virus (TYLCV)

Begomovirus

Geminiviridae

Co-IP

https://doi.org/10.1101/811091

2020

Turnip mosaic virus (TuMV)

Potyvirus

Potyviridae

Co-IP

29593293

2018

Turnip mosaic virus (TuMV)

Potyvirus

Potyviridae

Co-IP

28223439

2017

Vaccinia virus (VacV)

Orthopoxvirus

Poxviridae

Co-IP

28467896

2017

Varicella zoster virus

Varicellovirus

Herpesviridae

Co-IP

https://doi.org/10.1101/2020.03.12.988659

2020

There are more references from 2015 and older, which are not included in above table. You can find those and additional publications, in which GFP-Trap and other ChromoTek products have been applied to generate data, here: https://www.chromotek.com/references/

 

ChromoTek’s GFP-Trap® is a versatile tool for the immunoprecipitation (IP) of GFP-tagged proteins. It consists of an anti-GFP Nanobody that is covalently coupled to beads. For a product overview see this link https://www.chromotek.com/products/detail/product-detail/gfp-trap/.

Nanobody-Alpaca-Chromotek-Trap

The interaction between the Nanobody and GFP is extraordinarily stable and efficient. This allows using cell lysate originating from many different organisms that require a multitude of different IP conditions and protocols. Due to the GFP-Trap’s high affinity and low background, low abundance GFP-fusion proteins can be effectively pulled down too.

 

Try for yourself and request your sample:

GFP-Trap sample

 

 

Pictures: Many thanks for the virus picture to CDC on Unsplash

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