ChromoBlog

Which are 2018's most used Antibodies?

Posted by Klaus Herick on Aug 26, 2019 4:29:03 PM

CiteAB_Blog Post

 
ChromoTek’s GFP-Trap is again among the top 100 cited antibodies

 

CiteAb has analyzed more than 47,000 antibodies, which were cited in 2018 published literature. Their ranking has been announced in a recent blog . ChromoTek is honored that its GFP-Trap® is among the top 100 used antibodies in 2018, again after the 2017 analysis.

Goldstandard_Logo_small

ChromoTek’s literature analysis reveals a total of more than 1,600 scientific articles that are referencing GFP-Trap since its launch in 2008. This is awesome. The GFP-Trap is the most frequently cited monoclonal anti-GFP antibody and the gold standard for immunoprecipitation of GFP-fusion proteins. We are proud about so much trust in our products and proud to have helped our customers with the experiments for so many papers!

GFP-Trap consists of an anti-GFP VHH (Nanobody or single domain antibody) that is conjugated to a matrix, e.g. agarose or magnetic agarose. It is ready to use for immunoprecipitation, Co-IP for MS, ChiP/iCLIP/RIP, affinity purification, and enzymatic assay applications.

Using GFP-Trap you benefit from
  • No heavy & light chains from conventional IgG antibodies
  • Low background
  • Very high binding affinity with a dissociation constant of 1 picomolar
  • Pulldown of endogenous level expressed proteins from knock-ins like CRISPR/Cas
  • High specificity
  • Working in harsh wash conditions for detection of selective protein-protein interactions. Buffer components include high salt, denaturing & reducing reagents, glycerol, and detergents e.g. for pulldown of membrane proteins
  • Capable to work in large buffer volumes
  • Short incubation times of 30 minutes; extreme slow dissociation for prolonged incubations when needed

Hence, ChromoTek’s GFP-Trap is the best anti-GFP antibody for immunoprecipitation!

You may request a free test sample here: https://resources.chromotek.com/nano-trap-sample

ChromoTek and GFP-Trap are registered trademarks of ChromoTek GmbH. Nanobody is a registered trademark of Ablynx, a Sanofi company.

 

Request a free sample & test GFP-Trap

 

Topics: GFP-Trap, GFP Immunoprecipitation, GFP VHH, GFP Nanobody

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