ChromoBlog

Jellyfish Green Fluorescent Protein (GFP) and its derivatives are still the most frequently used fluorescent proteins in biomedical research. Recently, additional green fluorescent proteins have been discovered in higher animals such as crustaceans and lancelets. These FPs share a common fold, but diverge widely in their primary sequence. Thus, they require novel, dedicated antibody research tools. Here is an overview about EGFP (the most commonly used GFP derivative), TurboGFP and mNeonGreen.

 

EGFP
(enhanced GFP)

TurboGFP

mNeonGreen

Discovery/
first publication

1995

2004

2013

Structure

GFP

turboGFP

 

mNeonGreen

 

Origin

GFP from Jellyfish
Aequorea victoria

Tetrameric CopGFP from copepod Pontellina plumata

Tetrameric LanYFP from Lancelet Branchiostoma lanceolatum

Sequence identity to EGFP

(100%)

~20%

20-25%

Derived from GFP?

Yes

No

No

Structure

Monomer

Dimer

Monomer

Length/ molecular weight (MW)

239 amino acids,
26.9 kDa

2x 232 amino acids,
2x 26 kDa

237 amino acids,
26.6 kDa

Excitation / Emission maximum

488/ 509 nm

482 nm/ 502 nm

506 nm/ 517 nm

Common variants

GFP, CFP, YFP, Venus, BFP and many more

TurboGFP, CopGFP

mNeonGreen

Research Tools

IP
GFP-Trap

IF
GFP-Booster,
GFP antibody PABG1

WB
GFP antibody [3H9]
GFP antibody [PABG1]

Control
purified EGFP protein

IP
TurboGFP-Trap

IP
mNeonGreen-Trap

IF
mNeonGreen antibody [32F6]

WB
mNeonGreen antibody [32F6]

Shortcuts:
IP = Immunoprecipitation, IF = Immunofluorescence, WB = Western blot

Working with GFP, TurboGFP or mNeonGreen?

Doing immunoprecipitations?

Want to test one of the Nano-Traps for yourself?   Request your free sample

 

Topics: TurboGFP immunoprecipitation, GFP Immunoprecipitation, mNeonGreen immunoprecipitation, EGFP, GFP, mNeonGreen, TurboGFP, Nanobody

Subscribe to Email Updates

Recent Posts

Posts by Topic

see all