V5-Trap for immunoprecipitation (IP) of V5-tagged protein
Request your test sample (right column) and benefit from the V5-TrapTM and the Alpaca Antibody Advantage in your next IP:
- Very low background
- No heavy and light antibody chains
- High specificity, high binding capacity
- Extraordinarily stable, also under harsh washing conditions
- Compatible with N-, C-terminal and internal V5-tag
- Effective elution with V5-peptide
- Recombinant for consistent results
I: input, FT: flow-through, B: bound
ChromoTek’s V5-Trap consists of the first Nanobody against the V5-tag. The anti-V5 Nanobody is conjugated to agarose beads and magnetic agarose beads:
The Alpaca Antibody Advantage for quantitative Immunoprecipitations
Alpacas have unique antibodies, which consist of only heavy chains. As an exciting result the single antigen binding domain can be separately expressed: ChromoTek has developed the Nano-Trap utilizing the smallest intact antigen binding fragment. It is only 15 kDa in size, easy to handle and has ultra-high specificity and affinity to its respective antigen.
Western Blotting
For detection, use the extraordinarily sensitive and specific anti-V5 antibody [SV5-P-K].
The anti-V5 antibody [SV5-P-K] is a recombinant, monoclonal mouse IgG1, has very low background and detects even low amounts of V5-tagged proteins. It works great in combination with Alpaca anti-mouse IgG1 Nano-Secondary for detection.