One-step immunostaining with Nano-Secondaries

One-step staining protocol for immunofluorescence detection

Nano-Secondaries are monovalent and bind with high specificity and affinity to their target IgGs, they can be simultaneously incubated with the primary antibody. This results in a one-step immunostaining. It saves incubation time and reduces washing steps, and hand-on time. Simultaneous incubation also supports multiplexing, live-cell immunostaining, and improves cell viability for flow cytometric analysis.